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产品规格
检测灵敏度
0.16 ng/mL
测定范围
0.16-10 ng/mL
检测样本类型/体积
{Plasma=20 µL, Serum=20 µL, Supernatant=20 µL}
手工操作时间
24 hr
总时间
2 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture (coating) Antibody
Detection Antibody
Standard
Sample Diluent
Detection Antibody
Standard
Sample Diluent
运输条件
Wet ice
储存
-20°C
蛋白名称
CD154 (CD40 Ligand)
Target site-specificity
Target sub-specificity
Soluble
检测试剂盒格式
Matched Antibody Pair
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human CD40L Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human CD40L. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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