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![ERK1/ERK2 (Phospho) [pT202/pY204] ELISA Kit (EMS2ERKP)](https://www.thermofisher.cn/antibody/images/650/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826 "ERK1/ERK2 (Phospho) [pT202/pY204] ELISA Kit (EMS2ERKP)")
![ERK1/ERK2 (Phospho) [pT202/pY204] ELISA Kit (EMS2ERKP)](https://www.thermofisher.cn/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826)
产品规格
检测灵敏度
2.67 pg/mL
测定范围
62.5-2000 pg/mL
检测样本类型/体积
{Cell Lysate=12.5 µL}
手工操作时间
1 hr 20 min
总时间
3 hr
均相（无需洗涤）
No
批间差异
6.3%
批内差异
3.7%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Detection Antibody
Anti-Rabbit-HRP
Cell Lysis Buffer
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Detection Antibody
Anti-Rabbit-HRP
Cell Lysis Buffer
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Dry ice
储存
2-8°C
蛋白名称
ERK1/2
Target site-specificity
Phosphorylated Thr202, Phosphorylated Tyr204
蛋白家族
Other Proteins
均相（无需洗涤）
Human, Mouse, Rat
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The ERK1/2 [pThr202/Tyr204] ELISA quantitates IL-1b in cell lysates. The assay will exclusively recognize both natural and recombinant ERK 1/2, when phosphorylated at Threonine 202 and Tyrosine 204.
原理方法
The phospho ERK1/2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : 9030612K14Rik, AA407128, AU018647, C78273, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, Esrk1, HS44KDAP, HUMKER1A, Mapk, MAPK1, MAPK2, MAPK3, MNK1, Mtap2k, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM1, PRKM2, PRKM3
基因符号 : MAPK1, MAPK3, Mapk1, Mapk1, Mapk3, Mapk3
蛋白别名 : ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, extracellular-signal-regulated kinase 2, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Microtubule-associated protein 2 kinase, mitogen activated protein kinase 1, mitogen-activated 3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, MNK1, p42-MAPK, p44 MAP kinase, p44-ERK1, p44-MAPK, pp42/MAP kinase, protein tyrosine kinase ERK2
UniProt ID (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844
