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产品规格
检测灵敏度
50 pg/mL
测定范围
24.69-18,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
FGF4
蛋白家族
Growth Factors & Receptors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Fibroblast Growth Factor 4 (Hu FGF4) ELISA quantitates Hu FGF4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF4.
原理方法
The Human FGF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : FGF4, HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF, KS3
基因ID : (Human) 2249
基因符号 : FGF4
蛋白别名 : FGF-4, Fibroblast growth factor 4, fibroblast growth factor 4 splice isoform, HBGF-4, heparin secretory transforming protein 1, Heparin secretory-transforming protein 1, Heparin-binding growth factor 4, HST, HSTF-1, human stomach cancer, transforming factor from FGF-related oncogene, kaposi sarcoma oncogene, oncogene HST, Transforming protein KS3
UniProt ID (Human) P08620
