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Invitrogen
FT3 Competitive ELISA Kit
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产品规格
检测灵敏度
0.94 pg/mL
测定范围
1.56-100 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
FT3
蛋白家族
Thyroid hormone
均相（无需洗涤）
All
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The multispecies Free Triiodothyronine (fT3) ELISA quantitates fT3 in serum, plasma, and other biological fluids
原理方法:
The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Free Triiodothyronine (fT3) independent of species. A fT3 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to fT3 added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Free triiodothyronine (FT3) is a thyroid hormone that plays a critical role in regulating metabolism and energy production in the body. FT3 is produced by the thyroid gland and is responsible for increasing the metabolic rate of cells. It binds to nuclear receptors in the cell, activating transcription of genes involved in energy metabolism. FT3 also regulates the expression of genes involved in growth and development, making it essential for normal growth and development. Structurally, FT3 is a tyrosine-based hormone composed of three iodine atoms and is highly lipophilic. It circulates in the blood bound to plasma proteins and is transported to target tissues where it exerts its effects.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Free triiodothyronine
