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Invitrogen
Human Aggrecan (PG) ELISA Kit
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产品规格
检测灵敏度
0.9 ng/mL
测定范围
10-250 ng/mL
检测样本类型/体积
{Serum=20 µL, Supernatant=10 µL, Synovial Fluid=25 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 15 min
均相（无需洗涤）
No
批间差异
5.6%
批内差异
2.1%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample diluent
Incubation Buffer
HRP conjugated Detection Antibody
Conjugate Diluent
Wash Buffer
Chromogen
Stop Solution
Controls
Standard
Sample diluent
Incubation Buffer
HRP conjugated Detection Antibody
Conjugate Diluent
Wash Buffer
Chromogen
Stop Solution
Controls
运输条件
Wet ice
储存
2-8°C
蛋白名称
Aggrecan
蛋白亚型
Proteoglycans
蛋白家族
Other Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Aggrecan (PG) ELISA quantitates Hu Aggrecan (PG) in humansynovial fluid, serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Aggrecan (PG).
原理方法
The Human Aggrecan (PG) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second enzyme-conjugated (detector) antibody, binding to the target on a different epitope from the capture antibody. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : ACAN, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK
基因ID : (Human) 176
基因符号 : ACAN
蛋白别名 : Aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, large aggregating proteoglycan
UniProt ID (Human) Q9UCP4
