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Invitrogen
Human ICAM-3 (Soluble) ELISA Kit
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产品规格
检测灵敏度
0.38 ng/mL
测定范围
0.78-50 ng/mL
检测样本类型/体积
{Amniotic Fluid=20 µL, Plasma=20 µL, Serum=20 µL, Supernatant=20 µL, Urine=20 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 10 min
均相（无需洗涤）
No
批间差异
2%
批内差异
2.5%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
CD50 (ICAM3)
Target site-specificity
Target sub-specificity
Soluble
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Intercellular Adhesion Molecule-3 (Hu ICAM-3) ELISA quantitates Hu ICAM-3 in human serum, plasma, amniotic fluid, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ICAM-3.
原理方法
The Human ICAM-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : ICAM-3, CDw50, ICAM-R
