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Invitrogen
Human MMP1-Cell Lysate ELISA Kit
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产品规格
检测灵敏度
8 pg/mL
测定范围
8-18,000 pg/mL
检测样本类型/体积
{Cell Lysate=20 µL, Supernatant=40 µL, Plasma=20 µL, Serum=20 µL, Tissue Homogenate=20 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Cell Lysis Buffer
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Cell Lysis Buffer
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
MMP1
蛋白家族
Cell Structure/Organelle Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Matrix MetalloProteinase 1 (Hu MMP1) ELISA quantitates Hu MMP1 in human cell and tissue lysate. The assay will exclusively recognize both natural and recombinant Hu MMP1.
原理方法
The Human MMP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CLG, CLGN, MMP1
基因ID : (Human) 4312
基因符号 : MMP1
蛋白别名 : Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, Collagenase, matrix metallo protease, Matrix metalloproteinase1, MMP, MMPs, Interstitial collagenase, matrix metallopeptidase 1 (interstitial collagenase), matrix metalloprotease 1, matrix metalloproteinase 1
UniProt ID (Human) P03956
