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The Human MSP (MST1) ELISA quantitates Hu MSP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MSP.
原理方法
The Human MSP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
MSP (or MST1) contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : D3F15S2, DNF15S2, HGFL, MSP, MST1, NF15S2
基因ID : (Human) 4485
基因符号 : MST1
蛋白别名 : Hepatocyte growth factor-like protein, hepatocyte growth factor-like protein homolog, macrophage stimulating 1 (hepatocyte growth factor-like), Macrophage stimulatory protein, Macrophage-stimulating protein, MSP
UniProt ID (Human) P26927