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产品规格
检测灵敏度
<0.6 pg/mL
测定范围
4.69-300 pg/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL, Cell Lysate=50 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
3.4%
批内差异
2.1%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Biotinylated Detection antibody
Sample Diluent
Assay Buffer concentrate
Wash Buffer concentrate
SAV-HRP
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Biotinylated Detection antibody
Sample Diluent
Assay Buffer concentrate
Wash Buffer concentrate
SAV-HRP
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
PD-L1 (CD274)
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Programmed T cell Death Ligand 1 (Hu PD-L1) ELISA quantitates Hu PD-L1 in human serum, plasma, cell lysates or cell culture medium. The assay will exclusively recognize both natural and recombinant H PD-L1.
原理方法
The Human PD-L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : B7-H, B7H1, CD274, PD-L1, PDCD1L1, PDCD1LG1, PDL1
基因ID : (Human) 29126
基因符号 : CD274
蛋白别名 : B7 homolog 1, B7-H1, CD274, CD274 antigen, PD-L1, PDCD1 ligand 1, Programmed cell death 1 ligand 1
UniProt ID (Human) Q66RK1
