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Invitrogen
Human PSGL-1/CD162 (Soluble) ELISA Kit
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产品规格
检测灵敏度
1.0 U/mL
测定范围
1.56-50 U/mL
检测样本类型/体积
{Serum=15 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 10 min
均相（无需洗涤）
No
批间差异
6.6%
批内差异
3.2%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
CD162 (PSGL-1)
Target sub-specificity
Soluble
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human P-selectin Glycoprotein Ligand-1 (Hu PSGL-1/CD162) ELISA quantitates Hu PSGL-1/CD162 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PSGL-1/CD162.
原理方法
The Human PSGL-1/CD162 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CD162, CLA, PSGL-1, PSGL1, SELPLG
基因ID : (Human) 6404
基因符号 : SELPLG
蛋白别名 : CD162, cutaneous lymphocyte-associated associated antigen, P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, Cutaneous lymphocyte associated antigen, SELPG
UniProt ID (Human) Q14242
