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Invitrogen
Human TK1 ELISA Kit
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产品规格
检测灵敏度
37.5 pg/mL
测定范围
62.5-4,000 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
TK1
蛋白家族
Thymidine kinase
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Thymidine Kinase 1 (TK1) ELISA quantitates TK1 in serum, plasma, and other biological fluids
The Human Thymidine Kinase 1 (TK1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : TK1, TK2
基因ID : (Human) 7083
基因符号 : TK1
蛋白别名 : EC 2.7.1.21, KITH, thymidine kinase 1 soluble isoform, thymidine kinase 1, soluble, Thymidine kinase, cytosolic, thymidine kinase-1
UniProt ID (Human) P04183
