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Invitrogen
Human TfR/Transferrin R/CD71 Rapid ELISA Kit
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产品规格
检测灵敏度
0.23 ng/mL
测定范围
0.39-25 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Transferrin Receptor (CD71)
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human TFR1 (Transferrin Receptor 1) ELISA quantitates TFR1 in serum and plasma.
原理方法
The Human Transferrin Receptor 1 (TFR1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Transferrin Receptor 1 (TFR1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Transferrin Receptor 1 (TFR1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Transferrin Receptor 1 (TFR1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CD71, IMD46, p90, T9, TFR, TFR1, TFRC, TR, TRFR
基因ID : (Human) 7037
基因符号 : TFRC
蛋白别名 : CD antigen CD71, CD71, p90, T9, TR, transferrin receptor, Transferrin receptor 1, Transferrin receptor protein 1, unnamed protein product
UniProt ID (Human) Q1HE24
