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Invitrogen
Human proGRP ELISA Kit
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产品规格
检测灵敏度
9.38 pg/mL
测定范围
15.63-1,000 pg/mL
检测样本类型/体积
{Cell Lysate=100 µL, Tissue Homogenate=100 µL, Plasma=100 µL, Serum=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
proGRP
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Pro Gastrin Releasing Peptide (ProGRP) ELISA quantitates ProGRP in serum, plasma and other biological fluids.
原理方法
The Human ProGRP solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
ProGRP, short for Pro-gastrin-releasing peptide, is a precursor protein that gives rise to the gastrin-releasing peptide (GRP) hormone. It plays a role in various physiological processes, particularly in the regulation of neuroendocrine functions. Functionally, proGRP is involved in the control of cell growth, neurotransmission, and the regulation of the release of gastrointestinal hormones. It acts as a neuropeptide and can influence the secretion of gastrin and other gastrointestinal peptides. Additionally, proGRP has been implicated in the development and progression of certain cancers, particularly small cell lung cancer. Structurally, proGRP is a protein that consists of a chain of amino acids. It undergoes post-translational processing to generate the active form of GRP, which is a shorter peptide consisting of 27 amino acids. Localization-wise, proGRP is primarily produced in the nervous system, particularly in neurons of the central and peripheral nervous systems. It is also found in certain endocrine cells of the gastrointestinal tract and in some neuroendocrine tumors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : BN, GRP, GRP-10, preproGRP, proGRP
基因ID : (Human) 2922
基因符号 : GRP
蛋白别名 : bombesin, Gastrin-releasing peptide, GRP, neuromedin C, pre-progastrin releasing peptide, prepro-GRP, pro-grp, testicular tissue protein Li 103
UniProt ID (Human) P07492
