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The Levonorgestrel (LNG) ELISA quantitates LNG in serum, plasma, urine, dried fecal extracts, saliva, milk, or cell culture medium.
原理方法
The Levonorgestrel (LNG) Competitive ELISA research-use-only kit is designed to quantitatively measure levonorgestrel present in serum, plasma, saliva, urine, dried fecal samples, milk, and tissue culture media samples. This kit measures total LNG in extracted serum, plasma, milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Levonorgestrel (4-Estren-17alpha-Ethynyl-18-homo-17beta-ol-3-one (LNG) is a synthetic steroid analogue.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名 : C21H28O2