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产品规格
检测灵敏度
<2 pg/mL
测定范围
15.6-1000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr
均相（无需洗涤）
No
批间差异
7.1%
批内差异
6.5%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Incubation Buffer
Biotinylated Detection Antibody
Strepavidin-HRP conjugate
HRP diluent
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive plate covers
Standard
Standard Dilution Buffer
Incubation Buffer
Biotinylated Detection Antibody
Strepavidin-HRP conjugate
HRP diluent
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive plate covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
CCL3 (MIP-1 alpha)
蛋白家族
Chemokines & Receptors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Macrophage Inflammatory Protein-1 alpha/Chemokine Ligand 3 (Hu MIP-1α/CCL3) ELISA quantitates Hu MIP-1α in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIP-1α.
原理方法
The Human MIP-1αsolid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : CCL3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3
基因ID : (Human) 6348
基因符号 : CCL3
蛋白别名 : C-C motif chemokine 3, chemokine (C-C motif) ligand 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, PAT 464.1, SIS-beta, small inducible cytokine A3 (homologous to mouse Mip-1a), Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein
UniProt ID (Human) P10147
