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Invitrogen
Mouse Periostin (Postn) ELISA Kit
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产品规格
检测灵敏度
0.05 ng/mL
测定范围
0.05-16 ng/mL
检测样本类型/体积
{Plasma=2 µL, Serum=2 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
Periostin
蛋白家族
Other Proteins
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Periostin (Postn) ELISA quantitates Ms Periostin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Periostin.
原理方法
The Mouse Periostin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1(TGF beta 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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生物信息学
基因别名 : A630052E07Rik, OSF-2, Osf2, PLF, PN
基因ID : (Mouse) 50706
基因符号 : Postn
蛋白别名 : osteoblast specific factor 2 (fasciclin I-like), osteoblast-specific factor 2, periostin-like factor, PLF, PN, Osteoblast-specific factor 2, OSF-2, fasciclin I-like, Fasciclin-I like, MGC119510, MGC119511, OSF 2, osteoblast specific factor 2, RP11 412K4.1, RP11-412K4.1, POSTN, osteoblast-specific factor, unnamed protein product
UniProt ID (Mouse) Q62009
