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产品规格
检测灵敏度
Lot-specific
测定范围
Lot-specific
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
2 x 96 Tests
产品成分
This Rat TNF-alpha ELISA Kit includes: Capture Antibody, Detection Antibody, Protein Standard, and HRP-Conjugate.
运输条件
Ambient
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
TNF alpha
蛋白家族
Growth Factors & Receptors
均相（无需洗涤）
Rat
检测试剂盒格式
ELISA Development Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Rat TNF-alpha ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant rat TNF-alpha in a sandwich ELISA format. Using the ELISA protocol, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay rat TNF-alpha in approximately 200 ELISA plate wells.
This PeproTech TMB ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant proteins in a sandwich ELISA format. This TMB ELISA Development Kit contains enough material to assay the target cytokine in at least 200 ELISA plate wells. The TMB ELISA Buffer Kit (Product # 900-T00), sold separately, is recommended to be used in conjunction with this TMB ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
靶标信息
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : RATTNF, Tnf, TNF-alpha, Tnfa, Tnfsf2
基因ID : (Rat) 24835
基因符号 : Tnf
蛋白别名 : Cachectin, TNF-a, TNF-alpha, Tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2
UniProt ID (Rat) P16599
