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产品规格
检测灵敏度
2.97 pg/mL
测定范围
7.8-1000 pg/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL, Fecal Extract=50 µL, Urine=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<6%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
Testosterone
蛋白家族
Chromone
均相（无需洗涤）
Chemical
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The multispecies Testosterone ELISA with improved sensitivity quantitates testosterone in serum, plasma, dried fecal samples, or urine.
原理方法:
The Competitive ELISA research-use-only kit is designed to quantitatively measure testosterone independent of species. A testosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with donkey anti-sheep IgG antibodies. A monoclonal sheep antibodies and testosterone-peroxidase conjugate is added to the wells. The testosterone conjugate and any testosterone in the sample will compete to bind to the sheep testosterone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound testosteron-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of testosterone present in the sample.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : 17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone, Testosterone
