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Multispecies p70 S6 Kinase (Phospho) [pT389] InstantOne™ ELISA Kit
![Multispecies p70 S6 Kinase (Phospho) [pT389] InstantOne™ ELISA Kit](https://www.thermofisher.cn/antibody/images/650/p70S6k-phospho-tds.jpg?time=20220826 "Multispecies p70 S6 Kinase (Phospho) [pT389] InstantOne™ ELISA Kit")
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Multispecies p70 S6 Kinase (Phospho) [pT389] InstantOne™ ELISA Kit
Representative data for p70 S6 Kinase (Phospho) [pT389] Multispecies InstantOne™ ELISA. MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 70 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.
![Multispecies p70 S6 Kinase (Phospho) [pT389] InstantOne™ ELISA Kit](https://www.thermofisher.cn/antibody/images/150/p70S6k-phospho-tds.jpg?time=20220826)
![Multispecies p70 S6 Kinase (Phospho) [pT389] InstantOne™ ELISA Kit (85-86052-11)](https://www.thermofisher.cn/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20220826)
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产品规格
检测样本类型/体积
{Cell Lysate=20 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
运输条件
Wet ice
储存
2-8°C
蛋白名称
p70 S6 Kinase
Target site-specificity
Phosphorylated Thr389
均相（无需洗涤）
Human, Mouse
检测试剂盒格式
InstantOne ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human and mouse p70 S6K in cell lysates. Detection of rat p70 S6K is expected.The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
靶标信息
RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : 2610318I15Rik, 4732464A07Rik, 70kDa, AA959758, AI256796, AI314060, p70 S6KA, p70(S6K)-alpha, p70-alpha, p70-S6K, p70/85s6k, p70s6k, PS6K, RPS6KB1, S6K, S6K-beta-1, S6K1, STK14A
基因ID : (Human) 6198, (Mouse) 72508
基因符号 : RPS6KB1, Rps6kb1
蛋白别名 : 70 kDa ribosomal protein S6 kinase 1, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6 kinase, alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1, P70S6K1, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, ribosomal protein S6 kinase, 70kD, polypeptide 1, 70kDa, S6K, S6K-beta-1, serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A
UniProt ID (Human) P23443, (Mouse) Q8BSK8
