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Air-drying and Lyophilization for Assay |
Stabilizing diagnostic assays for reliable room-temperature storage and transport is critical for assay developers. Thermo Fisher Scientific offers the lyo-ready portfolio of glycerol-free enzymes and reagents optimized for lyophilization for excellent stability and cost-effective logistics. Additionally, our newest Invitrogen Dry-Ready Reagents simplify assay stabilization through a fast, affordable air-drying process that requires no specialized equipment, featuring a verified excipient mix to help ensure consistent results and accelerate assay development.
| Air-drying | Lyophilization | |
|---|---|---|
| Process: ~2 hours |  | Process: >24 hours |
| Equipment: Heating oven |  | Equipment: Lyophilizator |
| Product shipment and storage at room temperature |  | Product shipment and storage at room temperature |
| Excipients and manual included |  | Lyophilization service provider recommendation |
Air-drying technology
Experience the simplicity and efficiency of the air-drying process by gently heating materials at moderate temperatures in a basic ventilated oven. Explore our extensive range of Dry-Ready raw materials, specially formulated to support all your air-drying applications.
- Effortless air-drying: Dry-Ready reagents are optimized to withstand hot air-drying, streamlining assay preparation with a fast and reliable process
- Long-term stability: Reaction buffers fortified with excipients help ensure your dried assays remain stable even at elevated temperatures
- Maximum flexibility: Choose from stand-alone enzymes, nucleotides, and buffers to create customized, dryable assay master mixes tailored to your needs
Figure 1. Air-drying workflow. This simple, fast, and efficient process aims to streamline your drying workflow. Just combine all assay components according to the instructions, dispense into plates, tubes, or strips, and air dry in a ventilated heating oven. Once dried, the product remains stable at room temperature for at least 12 months.
Dry-Ready products
The Invitrogen Dry-Ready RT-qPCR Kit offers an innovative solution for preparing hot air-dried RT-qPCR assays. Leveraging advanced Invitrogen reagents, this simple and rapid air-drying method outperforms traditional lyophilization in speed and ease, while enabling stable room-temperature storage and shipment of assays.
| Product | Components |
|---|---|
| Dry-Ready RT-qPCR Kit | Dry-Ready Platinum II DNA Polymerase |
| Dry-Ready SuperScript III Flash Reverse Transcriptase | |
| Dry-Ready RNaseOUT Recombinant Ribonuclease Inhibitor | |
| Dry-Ready dNTP Mix | |
| 10X Dry-Ready Reaction Buffer | |
| MgCl2 |
Features of the Dry-Ready RT-qPCR Kit include:
- Complete components for assay drying and downstream RT-qPCR applications
- Dry-Ready SuperScript III Flash Reverse Transcriptase enables rapid reverse transcription in as little as 1 minute
- Dry-Ready Platinum II DNA Polymerase offers high reliability with increased inhibitor tolerance
- Enhanced stability specifically optimized for air-drying processes
The Dry-Ready RT-LAMP Kit is a comprehensive solution for air-drying RT-LAMP assays. Optimized for a simple drying process that helps preserve assay functionality, it offers flexibility in initial sample volume and enhances sensitivity—making it an exceptional choice for robust stabilization in point-of-care applications.
| Product | Components |
|---|---|
| Dry-Ready RT-LAMP Kit | Dry-Ready SuperScript IV Reverse Transcriptase |
| Dry-Ready Bst DNA Polymerase | |
| Dry-Ready dNTP Mix | |
| 10X Dry-Ready RT-LAMP Reaction Buffer |
Features of the Dry-Ready RT-LAMP Kit include:
- Complete set of components for assay drying and downstream RT-LAMP applications
- SuperScript IV Reverse Transcriptase for rapid RNA conversion
- Robust Bst DNA Polymerase engineered for fast, sensitive, and efficient amplification
Stability testing of the Dry-Ready RT-qPCR Kit demonstrated that air-dried assays retained full activity after 10 weeks of storage at 50°C using the XENO RNA template. Across four input copy numbers (2,000; 10,000; 50,000; and 200,000), the air-dried assay (yellow) showed no loss of activity or sensitivity compared to freshly prepared assays (blue), confirming its robustness and reliability for long-term storage at elevated temperatures.
Figure 2. Stability of Dry-Ready RT-qPCR Kit after air drying.
The Dry-Ready Excipient Mix stabilizes air-dried assays containing Dry-Ready SuperScript III Reverse Transcriptase and Dry-Ready Platinum Taq DNA Polymerase for at least 10 weeks at 50°C, as demonstrated with the XENO RNA template. Tested at two input levels (2,000 and 200,000 copies), the air-dried assay (yellow) showed no loss of activity or sensitivity compared to freshly prepared assays (blue), confirming its stability and reliability for long-term storage at elevated temperatures.
Dry-Ready Excipient is a customized product for OEM use. For custom and commercial supply needs, please contact us at thermofisher.com/MDxContact.
Figure 3. Stability of Dry-Ready Excipient Mix after air drying.
Stability data for the Dry-Ready RT-LAMP Kit show that air-dried assays retained full activity after 14 weeks of storage at 37°C as demonstrated with human Adenovirus41 DNA template. The air-dried assays (red) demonstrated no loss of activity or sensitivity compared to freshly prepared assays (blue), confirming their stability and reliability for long-term storage at elevated temperatures.
Figure 4. Stability of Dry-Ready RT-LAMP Kit after air drying.
The Invitrogen Dry-Ready RT-qPCR Kit offers an innovative solution for preparing hot air-dried RT-qPCR assays. Leveraging advanced Invitrogen reagents, this simple and rapid air-drying method outperforms traditional lyophilization in speed and ease, while enabling stable room-temperature storage and shipment of assays.
| Product | Components |
|---|---|
| Dry-Ready RT-qPCR Kit | Dry-Ready Platinum II DNA Polymerase |
| Dry-Ready SuperScript III Flash Reverse Transcriptase | |
| Dry-Ready RNaseOUT Recombinant Ribonuclease Inhibitor | |
| Dry-Ready dNTP Mix | |
| 10X Dry-Ready Reaction Buffer | |
| MgCl2 |
Features of the Dry-Ready RT-qPCR Kit include:
- Complete components for assay drying and downstream RT-qPCR applications
- Dry-Ready SuperScript III Flash Reverse Transcriptase enables rapid reverse transcription in as little as 1 minute
- Dry-Ready Platinum II DNA Polymerase offers high reliability with increased inhibitor tolerance
- Enhanced stability specifically optimized for air-drying processes
The Dry-Ready RT-LAMP Kit is a comprehensive solution for air-drying RT-LAMP assays. Optimized for a simple drying process that helps preserve assay functionality, it offers flexibility in initial sample volume and enhances sensitivity—making it an exceptional choice for robust stabilization in point-of-care applications.
| Product | Components |
|---|---|
| Dry-Ready RT-LAMP Kit | Dry-Ready SuperScript IV Reverse Transcriptase |
| Dry-Ready Bst DNA Polymerase | |
| Dry-Ready dNTP Mix | |
| 10X Dry-Ready RT-LAMP Reaction Buffer |
Features of the Dry-Ready RT-LAMP Kit include:
- Complete set of components for assay drying and downstream RT-LAMP applications
- SuperScript IV Reverse Transcriptase for rapid RNA conversion
- Robust Bst DNA Polymerase engineered for fast, sensitive, and efficient amplification
Stability testing of the Dry-Ready RT-qPCR Kit demonstrated that air-dried assays retained full activity after 10 weeks of storage at 50°C using the XENO RNA template. Across four input copy numbers (2,000; 10,000; 50,000; and 200,000), the air-dried assay (yellow) showed no loss of activity or sensitivity compared to freshly prepared assays (blue), confirming its robustness and reliability for long-term storage at elevated temperatures.
Figure 2. Stability of Dry-Ready RT-qPCR Kit after air drying.
The Dry-Ready Excipient Mix stabilizes air-dried assays containing Dry-Ready SuperScript III Reverse Transcriptase and Dry-Ready Platinum Taq DNA Polymerase for at least 10 weeks at 50°C, as demonstrated with the XENO RNA template. Tested at two input levels (2,000 and 200,000 copies), the air-dried assay (yellow) showed no loss of activity or sensitivity compared to freshly prepared assays (blue), confirming its stability and reliability for long-term storage at elevated temperatures.
Dry-Ready Excipient is a customized product for OEM use. For custom and commercial supply needs, please contact us at thermofisher.com/MDxContact.
Figure 3. Stability of Dry-Ready Excipient Mix after air drying.
Stability data for the Dry-Ready RT-LAMP Kit show that air-dried assays retained full activity after 14 weeks of storage at 37°C as demonstrated with human Adenovirus41 DNA template. The air-dried assays (red) demonstrated no loss of activity or sensitivity compared to freshly prepared assays (blue), confirming their stability and reliability for long-term storage at elevated temperatures.
Figure 4. Stability of Dry-Ready RT-LAMP Kit after air drying.
Ordering information
Lyophilization technology
Lyophilization removes water from substances by freezing and then sublimating the ice under reduced pressure. This process helps preserve product integrity, support a longer shelf life, and is widely used to stabilize sensitive diagnostic reagents. Thermo Fisher’s Lyo-Ready enzymes help maintain the key performance attributes of conventional enzymes—reproducibility, sensitivity, and specificity—essential for reliable molecular diagnostic assay development.
Benefits of lyo-ready enzymes
- Versatile adaptability: Compatible with advanced microfluidics applications, including point-of-care testing, single-cell analysis, and droplet-based techniques
- Comprehensive portfolio: Wide range of stand-alone enzymes, nucleotides, and glycerol-free buffers to optimize your assay workflows
- Sustainable supply chain: Lyophilization enables cost-effective logistics and reduces cold chain energy consumption by eliminating the need for dry ice during transport
Lyo-ready products
Enzyme adaptability
Cartridge-based technologies often feature narrow flow channels, and thanks to the low viscosity of Lyo-Ready enzymes, flow is designed to continue smooth and clog-free throughout the system.
Lyo-ready enzymes hold promise and potential for faster and accurate point of care diagnostics. According to an article published in Nature , these 'lyo-ready' enzymes can be used to build dried mixtures of PCR reagents that enable sample-to-answer assays. Thermo Fisher Scientific offers customized solutions for specific applications, including modified concentrations, volume, and packaging.
Portfolio of lyo-ready enzymes
Explore lyo-ready enzymes for reverse transcription, isothermal amplification, PCR amplification, and other related technologies.
| Product | Applicable technology |
|---|---|
| Lyo-ready SuperScript III Flash RT | 1-step RT-qPCR |
| Invitrogen Lyo-ready SuperScript Reverse Transcriptase, 1-step RT-qPCR | 1-step RT-qPCR |
| Invitrogen SuperScript III RT | 1-step RT-qPCR |
| Invitrogen SuperScript IV RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific Maxima H Minus RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific Maxima RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific M-MLV RT | 1-step RT-qPCR |
| Thermo Scientific RevertAid H Minus RT | 2-step RT-qPCR, 1-step RT-PCR, RT-LAMP, cDNA synthesis |
| Thermo Scientific RevertAid M-MLV RT | 1-step RT-qPCR |
| Product | Applicable technology |
|---|---|
| RPA proteins and reagents | RPA |
| Invitrogen Bst DNA Polymerase | LAMP, HAD, EXPAR, RCA, RPA |
| Thermo Scientific EquiPhi29 DNA Polymerase | MDA, WGA, RCA |
| Product | Applicable technology |
|---|---|
| Invitrogen Platinum II Taq DNA polymerase | 1-step RT-qPCR |
| Invitrogen Platinum Taq DNA Polymerase | 1-step RT-qPCR |
| Applied Biosystems AmpliTaq Gold DNA Polymerase | 1-step RT-qPCR |
| Invitrogen LibertyTaq DNA Polymerase | 1-step RT-qPCR |
| Thermo Scientific Phire Hot Start II DNA Polymerase | PCR |
| Product | Applicable technology |
|---|---|
| Invitrogen RNaseOUT RNase inhibitor | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis, NASBA, TMA |
| Thermo Scientific RiboLock RNase Inhibitor | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis, NASBA, TMA |
| Product | Applicable technology |
|---|---|
| Lyo-ready SuperScript III Flash RT | 1-step RT-qPCR |
| Invitrogen Lyo-ready SuperScript Reverse Transcriptase, 1-step RT-qPCR | 1-step RT-qPCR |
| Invitrogen SuperScript III RT | 1-step RT-qPCR |
| Invitrogen SuperScript IV RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific Maxima H Minus RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific Maxima RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific M-MLV RT | 1-step RT-qPCR |
| Thermo Scientific RevertAid H Minus RT | 2-step RT-qPCR, 1-step RT-PCR, RT-LAMP, cDNA synthesis |
| Thermo Scientific RevertAid M-MLV RT | 1-step RT-qPCR |
| Product | Applicable technology |
|---|---|
| RPA proteins and reagents | RPA |
| Invitrogen Bst DNA Polymerase | LAMP, HAD, EXPAR, RCA, RPA |
| Thermo Scientific EquiPhi29 DNA Polymerase | MDA, WGA, RCA |
| Product | Applicable technology |
|---|---|
| Invitrogen Platinum II Taq DNA polymerase | 1-step RT-qPCR |
| Invitrogen Platinum Taq DNA Polymerase | 1-step RT-qPCR |
| Applied Biosystems AmpliTaq Gold DNA Polymerase | 1-step RT-qPCR |
| Invitrogen LibertyTaq DNA Polymerase | 1-step RT-qPCR |
| Thermo Scientific Phire Hot Start II DNA Polymerase | PCR |
| Product | Applicable technology |
|---|---|
| Invitrogen RNaseOUT RNase inhibitor | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis, NASBA, TMA |
| Thermo Scientific RiboLock RNase Inhibitor | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis, NASBA, TMA |
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