Online tools for analyzing primers, setting up reactions, and performing biochemical conversions & calculations.
Primer design and analysis
Analyzes the Tm, MW and extinction coefficient of a primer, and gives dilution instructions.
Analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation etc.
Reaction setup calculators
PCR reaction setup with Thermo Scientific Phusion DNA Polymerases.
qPCR reaction setup with Thermo Scientific DyNAmo qPCR Kits.
Calculates DNA and RNA concentrations based on measured OD260 values.
Analyzes qPCR amplification efficiency based on the slope of the standard curve.
Calculates copy numbers for DNA originating from different organisms.
Compare the error rate of Thermo Scientific Phusion High-Fidelity DNA Polymerase with other DNA polymerases.