Multiplex Panel Capabilities for Real-Time PCR Workflows

Detect multiple targets in a single reaction

Multiplexing is a technique that allows for the simultaneous detection and differentiation of multiple target sequences in a single reaction using real-time PCR (qPCR). Multiplexing involves designing specific primers and probes for each target of interest and optimizing reaction conditions to enable accurate and reliable results. By eliminating the need for individual reactions for each target organism, multiplexing can help provide the advantages of saving time, resources, and costs. With a single reaction, multiple pathogens or genetic markers can be detected, enabling faster turnaround times and helping to streamline and optimize the productivity of your testing process.

Search multiplex panels by research area

Antimicrobial resistance and drug resistance research

Gastroenteric research

Nail fungal research

Respiratory research

Sexually transmitted infection (STI) research

Vaginal and women’s health research

Urinary tract infections research

Tick-borne pathogen research

Experimental design considerations

qPCR multiplexing, while advantageous, does come with its challenges. One of the main difficulties is the optimization of primer and probe combinations to enable the specific and efficient amplification of each target in the multiplex reaction. Another challenge lies in managing potential cross-reactivity or interference between different targets, which may require additional optimization steps to minimize false positives or inaccurate quantification. Careful experimental design and thorough validation are crucial to overcoming these challenges, helping to support the reliability and accuracy of multiplex qPCR results. 

Multiplexing with TrueMark real-time PCR panels

We offer pre-designed and qualified multiplex panels for detecting a broad range of infectious pathogens and resistance genes in research applications for vaginal / women’s health, respiratory infections, sexually transmitted infections, gastrointestinal infections, nail fungal infections, antimicrobial / drug resistance and more.

 

These panels are made to order and customizable to be reconfigured with your specified targets of interest:

  • Package sizes: 200 or 1,000 uL
  • Formats available for predesigned and qualified panels: Single tube, 96-well and 384-well pre-spotted plates

What’s the difference between testing levels for “pre-designed” and “qualified” panels?

 

 

Pre-designed panels

Qualified panels

In-silico analysis

 No high-risk oligo interactions identified 

Inclusivity (maximized strain coverage)

Additional functional testing

 Analytical sensitivity 

 

Linear dynamic range 

 

Competitive interference (if applicable) 

 

Antimicrobial resistance and drug resistance multiplex research panels

Panel name Beta-lactamase and Quinolone Resistance Carbapenemase and Beta-Lactamase Resistance Extended-Spectrum Beta-Lactamase and Quinolone Resistance
Multiplex ID Ba00000009_gmplx Ba00000011_gmplx Ba00000010_gmplx
Testing level   Pre-designed Pre-designed Pre-designed
Targets Beta-lactamase ampC1​ 
   
Carbapenem-hydrolyzing class A beta-lactamase KPC (blaKPC)​     
Cephalosporin-hydrolyzing class C beta-lactamase blaFOX | blaACC​     
Class A beta-lactamase SHV (blaSHV)​     
Class A extended-spectrum beta-lactamase CTX-M (blaCTX-M)​     
Class A extended-spectrum beta-lactamase PER​     
OXA-48 family class D beta-lactamase OXA (blaOXA)​     
Quinolone resistance pentapeptide repeat protein (QnrB)     
Quinolone resistance pentapeptide repeat protein QnrA | QnrS​     
Subclass B1 metallo-beta-lactamase VIM|NDM|IMP​     
Control Bacillus atrophaeus ​ 
Panel name Cephalosporin resistance: ESBLs Carbapenem resistance Carbapenem resistance: OXA-type Quinolone resistance
Multiplex ID Ba00000373_gmplx Ba00000374_gmplx Ba00000375_gmplx Ba00000389_gmplx
Testing level Qualified Qualified Qualified Qualified
Targets
  • CTX-M
  • GES / PER / VEB
  • SHV
  • TEM
  • IMP
  • KPC
  • NDM
  • VIM
  • OXA-23
  • OXA-48
  • OXA-51 / OXA-24
  • OXA-58
  • QnrA
  • QnrB
  • QnrS
Controls Bacillus atrophaeus
Panel name Macolide and tetracycline resistance Plasmid-mediated beta-lactamase resistance: AmpC and OXA-type Sulfonamides & trimethoprim resistance Vancomycin and beta-lactam resistance
Multiplex ID Ba00000402_gmplx Ba00000399_gmplx Ba00000400_gmplx Ba00000401_gmplx
Testing level Qualified Qualified Qualified Qualified
Targets
  • ermA / ermC
  • ermB
  • tetA / tetB
  • tetM
  • ACC / ACT / MIR
  • CMY / MOX
  • DHA / FOX
  • OXA-1 / OXA-2
  • dfrA1 / dfrA5 / dfrA12 / dfrA17
  • sul1 / sul2
  • mecA
  • mecC
  • vanA / vanA1 / vanB / vanC
Controls Bacillus atrophaeus

Gastroenteric multiplex research panels

Panel name Astrovirus, Adenovirus, ETEC (Enteric) Bacteroides, Enterovirus, Vibrio (Enteric and Environment) Sapovirus (Gastroenteritis) Yersinia, Clostridium difficile (Enteric)
Multiplex ID Pa00000012_gmplx Ba00000014_gmplx Vi00000018_gmplx Pa00000013_gmplx
Testing level   Pre-designed Pre-designed Pre-designed Pre-designed
Targets Adenovirus F40/41​       
Astrovirus​       
Bacteroides fragilis​       
Clostridium difficile toxin A/B      
Enterotoxigenic E. coli (ETEC​)       
Enterovirus​       
Sapovirus GII.5 / GII.8 / GIV.1 / GV​       
Sapovirus I / GII / IV​       
Vibrio cholerae / Vibrio parahaemolyticus / Vibrio vulnificus​       
Yersinia enterolitica​       
Control Bacillus atrophaeus 

 

 

Nail fungal multiplex research panels (with Bacillus atrophaeus control)

Panel name Candida Differentiation Dermatophyte 1

Fusarium, Trichophyton,

Aspergillus, Candida

 Non-
Dermatophyte 1
Multiplex ID Fn00000034_gmplx Fn00000030_gmplx Fn00000020_gmplx Fn00000033_gmplx
Testing level Pre-designed Pre-designed Pre-designed Pre-designed
Targets
  • Candida albicans​
  • Candida glabrata​
  • Candida parapsilosis​
  • Candida tropicalis
  • Epidermophyton floccosum
  • Microsporum
  • Trichophyton mentagrophytes complex 
  • Trichophyton soudanense
  • Aspergillus niger / Aspergillus terreus
  • Candida albicans / Candida parapsilosis /  Candida tropicalis / Candida glabrata​
  • Fusarium solani
  • Trichophyton spp
  • Alternaria spp. 
  • Aspergillus niger / Aspergillus terreus / Aspergillus flavus / Aspergillus fumigatus 
  • Fusarium solani
  • Scopulariopsis brevicaulis 

Nail fungal multiplex research panels (with RNase P control)

Panel name Candida Differentiation

Dermatophyte 1

 Fusarium, Trichophyton, Aspergillus, Candida Non-Dermatophyte 1
Multiplex ID Fn00000032_gmplx Fn00000029_gmplx Fn00000019_gmplx Fn00000031_gmplx
Testing level Qualified Qualified Qualified Qualified
Targets
  • Candida albicans​
  • Candida glabrata​
  • Candida parapsilosis​
  • Candida tropicalis
  • Epidermophyton floccosum 
  • Microsporum
  • Trichophyton mentagrophytes complex 
  • Trichophyton soudanense
  • Aspergillus niger / Aspergillus terreus
  • Candida albicans / Candida parapsilosis /  Candida tropicalis / Candida glabrata​
  • Fusarium solani
  • Trichophyton spp
  • Alternaria spp. 
  • Aspergillus niger / Aspergillus terreus / Aspergillus flavus / Aspergillus fumigatus 
  • Fusarium solani
  • Scopulariopsis brevicaulis 
Panel name Fusarium, Scopulariopsis, Candida - R Trichophyton, Candida, Aspergillus - R

Trichophyton, Candida, Fusarium - R

 Trichophyton, Candida, Scopulariopsis - R
Multiplex ID Fn00000269_gmplx Fn00000266_gmplx Fn00000267_gmplx Fn00000268_gmplx
Testing level Pre-designed Pre-designed Pre-designed Pre-designed
Targets
  • Candida albicans / Candida parapsilosis /  Candida tropicalis / Candida glabrata​
  • Fusarium solani
  • Scopulariopsis brevicaulis
  • Aspergillus niger / Aspergillus terreus
  • Candida albicans / Candida parapsilosis /  Candida tropicalis / Candida glabrata
  • Trichophyton soudanense
  • Candida albicans / Candida parapsilosis /  Candida tropicalis / Candida glabrata
  • Fusarium solani
  • Trichophyton soudanense
  • Candida albicans / Candida parapsilosis /  Candida tropicalis / Candida glabrata
  • Scopulariopsis brevicaulis
  • Trichophyton soudanense

Respiratory multiplex research panels

Panel name Bordetella differentiation (Whooping cough) Coronavirus differentiation FluA, FluB, FluA H5N1, SARS‑CoV‑2 Flu A, Flu B, RSV Parainfluenza differentiation
Multiplex ID Ba00000054_gmplx Vi00000053_gmplx Vi00000181_gmplx Vi00000052_gmplx Vi00000045_gmplx
Testing level   Qualified Qualified Qualified Qualified Qualified
Targets Bordetella holmesii        
Bordetella parapertussis​​         
Bordetella pertussis​​         
Coronavirus OC43        
Coronavirus HKU1        
Coronavirus 229E        
Coronavirus NL63        
Influenza A       
Influenza A H5N1        
Influenza B       
Parainfluenza 1​         
Parainfluenza 2​         
Parainfluenza 3​         
Parainfluenza 4        
RSV         
  SARS-CoV-2        
Control RNase P 
Panel name Legionella, C. burnetii, Group A Strep, P. jirovecii S. pneumoniae, H. influenzae, M. pneumoniae, Group B Strep S. aureus, M. catarrhalis, K. pneumoniae, C. pneumoniae
Multiplex ID Ba00000405_gmplx Ba00000403_gmplx Ba00000404_gmplx
Testing level Qualified Qualified Qualified
Targets
  • Coxiella burnetii
  • Legionella pneumophila / Legionella longbeachae
  • Pneumocystis jirovecii
  • Streptococcus pyogenes (Group A Streptococcus)
  • Haemophilus influenzae
  • Mycoplasma pneumoniae
  • Streptococcus agalactiae (Group B Streptococcus)
  • Streptococcus pneumoniae
  • Chlamydia pneumoniae
  • Klebsiella pneumoniae
  • Moraxella catarrhalis
  • Staphylococcus aureus
Control
 RNase P
Panel name Bocavirus, Paraechovirus, Flu C Metapneumovirus, Adenovirus Rhinovirus / Enterovirus
Multiplex ID Vi00000408_gmplx Vi00000406_gmplx Vi00000407_gmplx
Testing level Qualified Qualified Qualified
Targets
  • Human Bocavirus
  • Human Paraechovirus
  • Influenza C
  • Adenovirus
  • Human Metapneumovirus (hMPV)
  • Rhinovirus / Enterovirus
Control RNase P

Sexually transmitted infections (STI) multiplex research panels

Panel name Herpes Simplex
Multiplex ID Vi00000004_gmplx
Testing level Qualified
Targets
  • HSV 1
  • HSV 2
Control RNase P

Vaginal and women’s health multiplex research panels

Panel name Vaginal pathogen Vaginal profile Vaginal candida
Multiplex ID Pa00000001_gmplx Ba00000002_gmplx Fn00000041_gmplx
Testing level   Qualified Qualified Qualified
Targets Atopobium vaginae​     
Candida dubliniensis / Candida albicans / Candida parapsilosis / Candida tropicalis​   
Candida glabrata    
Candida glabrata / Candida krusei​     
Candida krusei​     
Gardnerella vaginalis​   
Lactobacillus jensenii / Lactobacillus crispatus / Lactobacillus gasseri​    
Megasphaera 1​     
Trichomonas vaginalis​     
Control RNase P​ 
Panel name Human Papillomavirus Low Risk 1 Human Papillomavirus Low Risk 2 Human Papillomavirus High Risk
Multiplex ID Vi00000395_gmplx Vi00000397_gmplx Vi00000396_gmplx
Testing level Qualified Qualified Qualified
Targets
  • HPV 31 / 35
  • HPV 52
  • HPV 58 / 33
  • HPV 39 / 59 / 68
  • HPV 51
  • HPV 56 / 66
  • HPV 16
  • HPV 18
  • HPV 45
Control RNase P

Urinary tract infections multiplex research panels

Panel name Urinary Tract Microbiota 1 Urinary Tract Microbiota 2 Urinary Tract Microbiota 3 Urinary Tract Microbiota 4
Multiplex ID Pa00000338_gmplx Pa00000339_gmplx Ba00000340_gmplx Ba00000273_gmplx
Testing level

Qualified
Targets

 		 Aerococcus urinae Candida auris Actinobaculum schaalii Acinetobacter baumannii
Candida albicans Candida parapsilosis Coagulase-negative staphylococci Klebsiella pneumoniae
Citrobacter freundii Escherichia coli Mycoplasma hominis   Morganella morganii
Candida glabrata Proteus mirabilis Providencia stuartii
Control Bacillus atrophaeus
Panel name Urinary Tract Microbiota 5 Urinary Tract Microbiota 6 Urinary Tract Microbiota 7 Urinary Tract Microbiota 8
Multiplex ID Ba00000342_gmplx Ba00000275_gmplx Ba00000343_gmplx Ba00000344_gmplx
Testing level

Qualified
Targets

 		 Alloscardovia omnicolens Corynebacterium riegelii Enterobacter aerogenes Pseudomonas aeruginosa
Citrobacter koseri Enterococcus faecalis Enterobacter cloacae Pantoea agglomerans
Enterococcus faecium Serratia marcescens Klebsiella oxytoca Proteus vulgaris
Ureaplasma urealyticum Viridans group streptococci Staphylococcus aureus Streptococcus agalactiae
Control Bacillus atrophaeus

Tick-borne pathogen multiplex research panels

Panel name Anaplasmosis and Ehrlichiosis Lyme Disease, Hard Tick Relapsing Fever Rickettsia, F. tularensis, C. burnetii Soft Tick Relapsing Fever
Multiplex ID Ba00000387_gmplx Ba00000385_gmplx Ba00000388_gmplx Ba00000386_gmplx
Testing level Qualified Qualified Qualified Qualified
Targets
  • Anaplasma phagocytophilum
  • Ehrlichia chaffeensis
  • Ehrlichia ewingii
  • Ehrlichia muris eauclairensis
  • Ixodida spp.
  • Borrelia burgdorferi
  • Borrelia lonestari
  • Borreliella mayonii
  • Borrelia miyamotoi
  • Coxiella burnetii
  • Francisella tularensis
  • Rickettsia philipii str. 364D
  • Rickettsia parkeri
  • Rickettsia rickettsii
  • Borrelia hermsii
  • Borrelia parkeri
  • Borrelia turicatae
  • Ixodida spp.
Panel name Babesiosis Tick-Borne RNA Virus 1 Tick-Borne RNA Virus 2
Multiplex ID Pr00000390_gmplx Vi00000391_gmplx Vi00000392_gmplx
Testing level Qualified Qualified Qualified
Targets
  • Babesia odocoilei
  • Babesia duncani
  • Babesia microti
  • Babesia MO1
  • Bourbon virus
  • Colorado tick fever virus
  • Deer tick virus
  • Heartland virus
  • Powassan virus
  • Crimean-Congo hemorrhagic fever virus
  • Dabie bandavirus
  • Tick-borne encephalitis virus

For Research Use Only. Not for use in diagnostic procedures.