Tomography 5 Software – a powerful, automated solution for 3D cryo-tomography

Cryo-electron tomography is a high-resolution technique that enables you to image a cell’s molecular machinery at physiological conditions. The in situ capabilities of cryo-tomography allow it to bridge the gap between single particle analysis (SPA) and structural cell biology, linking molecular insights to cellular behavior. Thermo Scientific Tomography 5 Software brings you the latest advances in cryo-tomography, allowing you to acquire valuable 3D data, even on your most challenging vitrified samples. Its new user-friendly interface enables both experienced and new users to jumpstart their tomography data acquisition.

Cryo-electron-tomography-workflow

Tomo Live Software is an optional add-on for Tomography 5 Software that offers automatic on-the-fly reconstruction with data curation for review -- in any browser, anytime, anywhere.

Tomography 5 Software user-friendly interface supports both experienced and new users to jump-start their tomography data acquisition.


Benefits

Reduced learning curve

Reduced learning curve

Reduced learning curve with step-by-step set-up of tomographic data acquisition.

Automated 3D data acquisition

Automated 3D data acquisition

Automated 3D data acquisition for multi-site tomography.

Intuitive features

Intuitive features

Intuitive featureshelp you set up regions for analysis and offer a range of imaging presets, such as feature-of-interest tracking.


Key Features

Multi-site batch tomography

Multi-site batch tomography for automated, 3D data acquisition, ensuring optimal cryo-transmission electron microscope (cryo-TEM) capacity. Navigate to several regions of interest and set up tomograms, which are automatically acquired. The fully automated data acquisition runs as soon as all settings are defined.

Multi-site batch tomography
Dose symmetric tilt scheme

Dose symmetric acquisition scheme

Dose symmetric acquisition scheme ensures optimal electron dose distribution and high resolution sub-tomogram averaging. During a tomographic tilt series, the acquisition scheme exposes the projections in the lower negative and positive angular tilt range uniformly, preserving high-resolution information. This collection scheme optimizes resolution (i.e., the sub-nanometer range) and provides considerably better results than continuous or bi-directional tilt schemes.

Automatic cassette mapping

Automatic cassette mapping delivers grid quality assessment and lamella identification. Map up to 12 grids stored in the cryo-TEM autoloader system. A low-magnification map of each grid is taken, allowing for grid quality assessment and efficient screening for cryo-lamella regions.

Automatic cassette mapping
Selectris Imaging Filter enabled

Selectris Imaging Filter enabled

The Thermo Scientific Selectris Imaging Filter greatly improves the signal-to-noise ratio (SNR) in tomography, boosting contrast and resolution through zero-loss filtering (i.e. the removal of inelastically scattered electrons). The imaging filter is fully integrated with Tomography 5 Software.

Cryo-ET automation with Tomo Live Software

Thermo Scientific Tomo Live Software is an optional add-on for Tomography 5 Software that offers on-the-fly data monitoring through real-time reconstruction of tilt series into 3D volumes. It can automatically perform motion correction of fraction movies, fiducial-free tilt series alignment, and 3D reconstruction during tilt series recording. These reconstructed tomograms allow for impromptu data quality evaluation, identification of high- and low-quality tilt series, and exporting of selected tilt series and reconstructed tomograms for further processing, archiving, and sharing.

Tomo Live Software runs on the microscope’s Data Management Platform server, and the user interface is integrated in the Thermo Scientific Athena™ Software portal.

Cryo-ET data workflow with Tomo Live Software
  • Automatic reconstruction
  • Combine 100% accurate and complete metadata from Thermo Scientific TEMs with smart estimate and parameter refinement for fully automatic 3D reconstruction within 5 minutes of a tilt series recording.

  • Curation
  • Tomo Live Software supports data curation by displaying quality metrics alongside the reconstructed tomograms. Define rulesets (e.g., alignment accuracy) that are applied during data export.

  • Export
  • Curated tomograms can be exported in a number of formats and are ready for segmentation in Thermo Scientific Amira™ Software. The motion-corrected and aligned tilt series are helpful for particle picking and sub-tomogram averaging workflows.

Curation

Service and technical support

Software Deployment

Deployment

We ensure seamless integration of Tomography 5 Software into your own on-premises environment, allowing you to optimize your infrastructure investments.

Techniques

冷冻电子断层扫描

冷冻电子断层扫描(cryo-ET)既能够提供单个蛋白的结构信息,又能够提供它们在细胞内的空间排列信息。这使该方法成为一种真正独特的技术,在细胞生物学领域具有巨大的潜力。冷冻ET可以将光学显微镜和近原子分辨率技术(如单颗粒分析)结合在一起。

了解更多 ›

整合结构生物学

要了解蛋白质功能,您需要单个蛋白质之外的复杂和结构信息。整合结构生物学将质谱分析法和冷冻电镜(Cryo-EM)结合起来用于大型动态复杂结构的确定。

了解更多 ›

冷冻电子断层扫描

冷冻电子断层扫描(cryo-ET)既能够提供单个蛋白的结构信息,又能够提供它们在细胞内的空间排列信息。这使该方法成为一种真正独特的技术,在细胞生物学领域具有巨大的潜力。冷冻ET可以将光学显微镜和近原子分辨率技术(如单颗粒分析)结合在一起。

了解更多 ›

整合结构生物学

要了解蛋白质功能,您需要单个蛋白质之外的复杂和结构信息。整合结构生物学将质谱分析法和冷冻电镜(Cryo-EM)结合起来用于大型动态复杂结构的确定。

了解更多 ›

Related Products

Krios G4 Cryo-TEM
Glacios 冷冻 TEM
Talos Arctica Cryo-TEM
Selectris


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