Analyzing Anions by Ion Chromatography

Anion analysis in food and beverage products

Weak-acid preservatives are anions that are widely used to prevent microbial spoilage of acidic foods and beverages, such as soda, fruit juice, soy sauce, jams, and other condiments. Inhibition by weak acids involves rapid diffusion of undissociated molecules through the plasma membrane, which liberates protons and acidifies the cytoplasm, preventing growth of microbes. Preservatives are more effective at low pH values where solutions contain increased concentrations of undissociated acids.

Anion analysis

Using a Thermo Scientific™ Reagent-Free™ ion chromatography (RFIC™) system simplifies weak-acid analysis, enabling full control of the hydroxide eluent concentration through Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software. Samples only require a simple dilution prior to injection, and precision is significantly improved by avoiding manual preparation of eluents.

Regulatory information

  • FDA
    • Requires labeling of any food or beverage containing a sulfite concentration over 10 ppm.
    • Lists benzoate as a substance that is generally recognized as safe (GRAS), with a maximum permitted concentration of 0.1% in accordance with good manufacturing or feeding practices.
    • Requires infant formulas to have a minimum iodine concentration of 5μg/100 kcal and no more than 75μg/100 kcal.
  • European Union
    • Established a benzoate limit of 0.015% in soft drinks and up to 0.2% in other food products.

Sampling of Application notes

Application NoteSampleIC Column
Rapid Determination of Phosphate and Citrate in Carbonated Soft Drinks Using a Reagent-Free™ Ion Chromatography System (AN 169)Soft drinksDionex IonPac Fast Anion III Columns
Determination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography (AN 165)Soy sauceDionex IonPac AS18 Columns
Determination of Total and Free Sulfite in Foods and Beverages (AN 54)WineDionex IonPac ICE-AS1 Columns
Determination of Iodide and Iodate in Soy- and Milk-based Infant Formulas (AN 37)Milk and Infant FormulaDionex IonPac AS11 Columns
Determination of fluoride in sodium fluoride oral solution using carbonate/bicarbonate eluent (AN 2344)FluorideIonPac AS22
Determination of fluoride in sodium fluoride oral solution (AN 2345)FluorideIonPac AS22
Application - The product is For General Lab Use Only - Not For Diagnostic Procedures

Our General Purpose product-lines are not intended for in vitro diagnostic purposes in accordance with our product documentation, manuals, and labels. They are designated for General Laboratory Use Only.

Our Products have not been tested or validated for such applications and their use for in vitro diagnostic purposes may result in inaccurate results and, more seriously, health and safety risks.

 

The product is For General Lab Use Only - Not For Diagnostic Procedures. The application is For Research Use Only. Not for use in diagnostic procedures.

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