GeneMapper PG Software

Mixtures meet their match with a simpler, smarter, all-in-one mixture interpretation solution

Advancements in DNA extraction, STR chemistry, and capillary electrophoresis have dramatically increased the sensitivity of forensic testing, enabling the recovery of usable DNA from more samples than ever before. With increased sensitivity and the high demand for DNA evidence, complex mixture interpretation continues to pose one of the greatest challenges in forensic DNA analysis.

 

GeneMapper PG Software extends the trusted GeneMapper ID-X Software with a seamless, intuitive suite of software designed to bring clarity, deeper insight, and confidence to your mixture analysis. With GeneMapper PG Software, transparency isn’t just a feature—it’s the foundation. With visualizations and clear explanations, you can track the logic, compare models, and be confident in your results.

  • Step-by-step logic and visualization tools provide rationale for each decision, streamlining validation and strengthening expertise
  • Multiple models for data-driven number of contributor (NoC) estimations
  • A fully continuous probabilistic model for precise, reproducible mixture deconvolution
  • Likelihood ratio calculation tools for scenarios ranging from simple to complex
  • Robustness analysis test simulated profiles to determine the strength of the person of interest (POI) likelihood ratio

Profile review

Start your interpretation with a big-picture view of your entire project. In the Profiles screen, manage your data for single or multiple cases within one project. Here you will find an evidence and reference list, allele table, built-in electropherogram views, and sample quality information. Within this screen, you can evaluate sample quality using built-in flags such as degradation and low peak height, group samples via custom tags, combine replicate amplifications into a single sample for analysis, and set initial (analyst evaluated) and final NoC results (post-Number of Contributors analysis) for each sample.

Number of contributors

Estimating the NoC is foundational to interpretation, informing every subsequent step. GeneMapper PG Software offers a variety of algorithms to determine the number of contributors in a DNA mixture. These algorithms range from basic methods like maximum allele count (MAC) to machine learning-based decision trees. Each method provides complete transparency, allowing you to scrutinize the estimation process in detail.

Deconvolution

Modeling biological aspects of forensic DNA profiles with customizable settings for locus, laboratory, STR kit, and instrument-specific values, the mixture deconvolution step employs the biological context optimized sampling (BCOS) algorithm. This deterministic algorithm follows a framework and fixed set of rules, for efficient and consistent analysis.

The software's powerful visualization tools and real-time explanations enable detailed analysis and exploration. Instantly view electropherograms to compare observed DNA profiles with expected solutions, extract major contributors, and export CODIS-compatible files for database searches.

Compare profiles

Whether examining pattern cases or tracking QC trends, profile comparison tools streamline data analysis across projects and cases. Heat maps highlight similarities and discrepancies across profiles, while a Match Table presents a list of best matches filtered by percentage to quickly identify the most relevant profiles.

Likelihood ratio calculations

Calculate even the most complex likelihood ratio (LR) scenarios. Build the scenario, visualize the result, and drill down into the calculation details. The powerful LR Robustness tool simulates thousands of profiles, configurable to be either unrelated or a specific family relationship to the POI, to produce an empirical cumulative distribution plot of likelihood ratios. By positioning your POI’s LR against these simulations, determine whether your LR result is significant or is possibly the result of a fortuitous match.

Human Identification Professional Services

HID professional services enable agencies to overcome critical implementation challenges through a collaborative partnership with Thermo Fisher, a worldwide leader in validated human identification solutions.

Professional services are available to support:

  • New laboratory development, implementation, and validation
  • Instrumentation and software integration
  • New protocol validation and testing
  • Workflow, software, and DNA theory training and educational services

Validation

Thermo Fisher Scientific validated GeneMapper PG Software v1.0 following the FBI Quality Assurance Standards (July 1, 2025) and guidelines from the Scientific Working Group on DNA Analysis Methods (July 1, 2025). Each laboratory using GeneMapper PG Software v1.0 should perform its own internal verification or validation studies to establish interpretation criteria and demonstrate that the software is appropriate and fit for its own human identification uses.

Ordering information

For Research, Forensic, Paternity and Cell Line Authentication. Not for use in Diagnostic and Therapeutic applications.