NovaFluor Yellow 755 excitation shown as dotted line and emission shown as solid red histogram
3561780/60552755(in buffer) 4
flow cytometry

NovaFluor Yellow 755 dye is excited by the yellow-green laser (561 nm) and is optimized for use in cell surface applications. It can be used for both spectral and conventional flow cytometry. With its distinct emission spectrum, NovaFluor Yellow 755 fits in a spectral space that is currently only accessible using cross-laser excited tandem dyes, such as PE-Cyanine7. Replacing highly cross-laser excited dyes may reduce spread, allowing for better resolution of low expressed markers. NovaFluor Yellow 755 dye should generally be paired with moderately expressed antigens to minimize spectral spillover in complex multicolor panels.

The macromolecule-based NovaFluor Yellow 755 dye produces stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

Thermo Fisher Scientific offers NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Yellow 755 primary antibodies

Benefits of NovaFluor Yellow 755 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Should be paired with moderately expressed antigens to minimize spillover
  • Can be used to replace cross-laser excited PE-Cyanine7
  • Produces stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Yellow 755 dye

Spectral signature of NovaFluor Yellow 755 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 755 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
 

Staining of mouse splenocytes with NovaFluor Yellow 755 conjugated antibody. C57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody (OKT3), Super Bright 436 only (left) or along with CD45 Monoclonal Antibody (HI30), NovaFluor Yellow 755 (right). Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation.
 

Staining of human PBMCs with NovaFluor Yellow 755 conjugated antibody. Normal human peripheral blood cells (PBMCs) were stained with CD19 Monoclonal Antibody (HIB19), eFluor 450 only (left) or with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 755 (right). Cells in the lymphocyte gate were used for analysis.

NovaFluor dyes

NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

Additional resources

Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.

Brilliant Ultra Violet™ 和 Brilliant Violet™ 是 Becton, Dickinson 公司或其附属公司的商标或注册商标，经许可使用。

仅供科研使用，不可用于诊断目的。