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R-藻红蛋白 (R-PE)
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| 5 | 488-561 | 575/26 | 565 | 576 | （缓冲液中）2 | 流式细胞仪 |
R-藻红蛋白（R-PE）是从红藻中分离出来的强荧光藻胆蛋白，具有极高的量子产额，可发出明亮的橙红色荧光。在实际应用中，R-PE 共轭物的灵敏度通常比相应的荧光素共轭物高 5 到 10 倍。此外，R-PE 还具有以下特性：
- 长波长荧光发射
- 大斯托克斯位移
- 蛋白质主链具有抗猝灭性
- 水溶性极高
- 结构均匀，分子量确定
R-PE 是一种用于荧光检测的大分子，主要用于流式细胞术、微阵列检测、酶联免疫吸附试验和其他需要高灵敏度但无光稳定性的应用。我们提供广泛的 R-PE 产品，包括一抗，二抗，链霉亲和素以及串联偶联物。
图1.流式细胞仪直方图显示用抗 CD4 抗体与 Invitrogen R-藻红蛋白 Zenon 标记试剂复合染色的人单核细胞。
图2.显示 CD4 APC 阳性细胞的流式细胞分析散点图。正常人体外周血细胞在未经刺激（左）或经细胞刺激混合液（加蛋白转运抑制剂）刺激后（右）。细胞内染色采用抗人CD4 APC 和抗鼠 IgG2a K同种型对照PE 或抗人 L-2 PE。通过可固定活细胞染料 eFluor 780 确定的淋巴细胞门内的活细胞用于分析。
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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