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GeneArt Protein Expression and |
The GeneArt gene-to-protein advantage
Expand your capacity and expedite your protein and antibody discovery workflow. We've combined our R&D breakthroughs in gene synthesis, sequence optimization, and expression systems for services that can function as a flexible extension of your lab. Outsource all or part of your protein expression and purification projects at scales supporting early discovery work through pilot studies and all the way up to 100 L+ production.
Advantages of selecting GeneArt Services:
- Higher yields—GeneOptimizer algorithm and ExpiCHO, Expi293, or Expi293 PRO expression systems
- Expert support—from consultation to ordering to delivery
- End-to-end speed—streamlined, fast production from gene synthesis to protein purification
| Development stages supported |  | ||||
| Service | HTP Antibody Expression | Mid-scale expression | Large-scale expression | Patheon Pharma Services | |
| Target yields* | 100 µg–3 mg | >10 mg | >1 g | Kilograms | |
| Expression volumes | 2.5 or 3 mL | 35–300 mL | 0.5–1 L | 2–25+ L | Cell Line Development |
| Standard production time** | Expi293 PRO: starting at 14 business days ExpiCHO or Expi293: starting at 15 business days | Defined in Statement of work | |||
| Express antibody service** | 12–13 business days | ||||
| Ideal for | Mass spectrometry, functional testing, structural studies, and further characterization | Toxicity and safety, preclinical planning | Therapeutic grade | ||
*Target yields are approximate for the expression volumes and depend on variables including exact sequence and vector
** All production times include gene synthesis
Express antibody service
Now you can get standard human IgG antibodies shipped in just 12–13 business days with our express antibody service. Compare options below to choose the right service for your needs.
| Express antibody service | Standard protein expression service | ||
|---|---|---|---|
| Protein type | Human IgG | Standard to complex antibodies and antibody derivatives; other proteins | |
| Available expression systems | Gibco ExpiCHO expression system Gibco Expi293 expression system Gibco Expi293 PRO expression system | ||
| Scales | 50 mL or 200 mL (Expi293) 50 mL or 250 mL (ExpiCHO) 35 mL (Expi293 PRO) | 2.5 mL–25+ L | |
| Vectors | Direct gene synthesis vectors only (standard or custom onboarded with prior expression project) | Direct gene synthesis or subcloning vectors (standard or custom) | |
| LC:HC ratio | ExpiCHO 1:1 Expi293 1:1 Expi293 PRO 2:1 | Customizable chain ratio | |
| Purification | Protein A affinity purification |
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| Documented analytical data options include:
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| Production time | 12–13 business days | Starting at 15 business days | |
Request a quote for protein expression services
Optimized protein expression
GeneArt Services help maximize protein expression and cost efficiency by optimizing at every step. It starts on a foundation of sequence optimization, choosing codon sequences customized for the desired expression system. Compound that with media and cells that allow for higher cell densities, high per-cell productivity, and high-efficiency transfection for expression yields up to 3 g/L. Taken together, the result is higher protein yields from the same culture volume.
Figure 1. Cloning and protein expression workflow using GeneArt Gene Synthesis services. Our workflows are available (A) without or (B) with proprietary GeneOptimizer software to optimize wild type sequences to improve cloning efficiencies and protein expression levels.
Learn how the GeneOptimizer algorithm was developed to improve protein expression
Our protein expression and purification services utilize commercially available Gibco ExpiCHO, Expi293, and Expi293 PRO Expression systems, enabling you to seamlessly work between your bench and our productions labs.
Expert project support
From start to finish, every protein project is powered by a team of expert technical project managers. Whether you know exactly what you need or would prefer consultation to help achieve your goals, support is tailored for your specific requirements. Benefit from our experience to help accelerate your standard expression projects and provide guidance for more complex protocols, when needed.
Saving time with protein expression outsourcing: A case study
A clinical-stage biotechnology company pioneering the development of bi-functional fusion proteins required the fast generation of research grade protein candidates for screening and lead selection.
- >400 unique Fc-fusion protein sequences were sent to Thermo Fisher Scientific, converted to DNA sequences, and codon-optimized for species of interest
- Synthesized genes were cloned into expression vectors for transient expression using the Gibco Expi Expression System, and proteins were purified using affinity chromatography
- Our customer received both the proteins and DNA for current validation work and any future needs
Value delivered by GeneArt protein expression services
- Generated a library of Fc-fusion protein candidates for in vitro, in vivo screening, and lead selection that supported IND filings to move therapeutics from bench to clinic
- Custom products delivered in 6 weeks from concept to compound, helping to save 6 monthsof antibody development in-house
Dive deeper into this example by watching the on-demand webinar
Ready to request a quote or reach out to our project team? Start here
Resources
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Support
Request more information on GeneArt Services
Order support:
Geneartpmo@thermofisher.com
Contact us:
North America and Asia: 1-800-955-6288
Europe: +49 (0)941 942 76-100
仅供科研使用，不可用于诊断目的。
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