The Genome Editing Resource Library provides you access to different educational and technical learning materials, including webinars, videos, application notes, validated protocols, scientific posters, and publications.

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TypeNameGenome editing technology
Application noteA rapid method for GFP tagging of genes and enrichment of edited cells CRISPR
Application noteAchieve high specificity and on-target efficiency with TrueCut HiFi Cas9 Protein   CRISPR
Application noteAdvancing CAR T cell therapy with CTS TrueCut Cas9 ProteinCRISPR
Application noteAnalysis of the performance of GMP-manufactured Cas9 nucleases for cell therapy applicationsCRISPR
Application noteAchieve functional knockout in up to 90% of human primary T cells CRISPR
Application noteDelivery solutions for immune cells  CRISPR
Application noteGene modification for cell therapy applications: Achieving efficiency with CTS TrueCut Cas9 Protein and the CTS Xenon SystemCRISPR
Application noteHow to monitor and minimize off-target events during genome editingCRISPR
Application noteImproving and simplifying the design of knock-in experiments  using the TrueDesign Genome EditorCRISPR, TALEN
Application noteImprove genome editing outcomes in biologically relevant cell modelsCRISPR, TALEN
Application noteKey considerations in genome editing  CRISPR, TALEN
Application notePowerful loss-of-function screening with LentiArray CRISPR gRNAs: maximize knockout efficiency while maintaining specificity CRISPR
Application noteUsing Sanger sequencing to facilitate CRISPR and TALEN-mediated genome editing workflows CRISPR, TALEN
ArticleCRISPR-generated CAR-T cells: A new frontier in the treatment of solid tumors  CRISPR
ArticleCollaboration and CRISPR tools move scientists closer to a cure for sight loss  CRISPR
ArticleDesign your CRISPR gene editing experiments in as little as five minutes  CRISPR, TALEN
ArticleLooking to overcome challenges in your gene editing workflow?  CRISPR, TALEN
ArticleModeling rare diseases using CRISPR gene editing—Could fruit flies save lives?  CRISPR
ArticleRevolutionizing drug discovery: The role of CRISPR gene editing in target validation  CRISPR
BrochureCRISPR-Cas9 Protein for Genome Editing—Getting Started Guide  CRISPR
BrochureCRISPR Genome Editing Tools  CRISPR
FAQsCRISPR-based genome editing—Getting Started FAQsCRISPR
FAQsCRISPR-based genome editing—Troubleshooting FAQsCRISPR
FAQsTALEN-based genome editing—Getting Started FAQsTALEN
FAQsTALEN-based genome editing—Troubleshooting FAQsTALEN
HandbookGenome Editing Resource GuideCRISPR, TALEN
InterviewCRISPR-Cas9: A transformative tool for treating debilitating genetic diseaseCRISPR
InterviewHow CRISPR provides a unique opportunity to generate rare disease avatarsCRISPR
InfographicCas9 Plasmids vs ProteinsCRISPR
InfographicCRISPR-Cas9 - A promising tool for therapeutics developmentCRISPR
InfographicTips & Tricks for CRISPR SuccessCRISPR
ProtocolCRISPR-Cas9 Genome Editing In Human Induced Pluripotent Stem CellCRISPR
ProtocolCell-Line Specific CRISPR RNP Transfection Conditions Using Lipofectamine CRISPRMAX ReagentCRISPR
ProtocolCell-Line Specific CRISPR RNP Electroporation Conditions Using Neon SystemCRISPR
ProtocolEmbryo Microinjection With CRISPR-Cas9 in Mice and ZebrafishCRISPR
ProtocolGeneral CRISPR RNP Transfection GuidelinesCRISPR
ProtocolGuidelines For Clone Isolation and ValidationCRISPR
ProtocolVerification of CRISPR Gene Editing EfficiencyCRISPR
Publication (2023)High-Efficiency CRISPR/Cas9-Mediated Correction of a Homozygous Mutation in Achromatopsia-Patient-Derived iPSCs  CRISPR
Publication (2023)Target-seq: single workflow for detection of genome integration site, DNA translocation and off-target events  CRISPR, TALEN
Publication (2022)Non-viral, specifically targeted CAR-T cells achieve high safety and efficacy in B-NHL  CRISPR, TALEN
Publication (2020)A Novel Screening Approach for the Dissection of Cellular Regulatory Networks of NF-κB Using Arrayed CRISPR gRNA Libraries  CRISPR
Publication (2020)Development of a Proline-Based Selection System for Reliable Genetic Engineering in Chinese Hamster Ovary Cells  CRISPR, TALEN
Publication (2019)Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells  CRISPR
Publication (2018)Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene Targeting  CRISPR
Publication (2018)TEG-seq: an ion torrent-adapted NGS workflow for in cellulo mapping of CRISPR specificity  CRISPR
Publication (2017)Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA  CRISPR
Publication (2017)Programmable type III-A CRISPR-Cas DNA targeting modules  CRISPR
Publication (2016)Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNACRISPR
Publication (2016)Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX CRISPR
Publication (2015)Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. CRISPR
Publication (2015)Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. CRISPR
Publication (2015)Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease CRISPR
Publication (2015)Efficient Intracellular Delivery of Native Proteins CRISPR
Publication (2015)CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning. CRISPR
Publication (2015)Engineered CRISPR-Cas9 nucleases with altered PAM specificities CRISPR
Publication (2015)Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers CRISPR
Publication (2015)Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells CRISPR
Publication (2015)In vivo genome editing using Staphylococcus aureus Cas9 CRISPR
Publication (2015)Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors CRISPR
Publication (2015)Multiplex conditional mutagenesis using transgenic expression of Cas9 and sgRNAs CRISPR
Publication (2015)Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells CRISPR
Publication (2015)A Cas9–guide RNA complex preorganized for target DNA recognition CRISPR
Publication (2014)Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases CRISPR
Publication (2014)Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects CRISPR
Publication (2014)DNA interrogation by the CRISPR RNA-guided endonuclease Cas9 CRISPR
Publication (2014)Programmable RNA recognition and cleavage by CRISPR/Cas9 CRISPR
Publication (2014)Landscape of target: guide homology effects on Cas9-mediated cleavage CRISPR
Publication (2014)Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA CRISPR
Publication (2014)Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter CRISPR
Publication (2014)Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo  CRISPR
Publication (2014)Guide RNA functional modules direct Cas9 activity and orthogonality CRISPR
Publication (2014)Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage CRISPR
Publication (2014)Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool CRISPR
Publication (2014)GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases CRISPR
Publication (2014)Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation CRISPR
Publication (2014)Improving CRISPR-Cas nuclease specificity using truncated guide RNAs CRISPR
Publication (2014)Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease CRISPR
Publication (2014)High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells CRISPR
Publication (2014)CRISPR-mediated direct mutation of cancer genes in the mouse liver CRISPR
Publication (2014)Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex CRISPR
Publication (2014)Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation CRISPR
Publication (2014)High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs CRISPR
Publication (2014)CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences CRISPR
Publication (2014)Genome-Scale CRISPR-Cas9 knockout screening in human cells CRISPR
Publication (2014)Genetic screens in human cells using the CRISPR-Cas9 system CRISPR
Publication (2014)Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells CRISPR
Publication (2014)Dimeric CRISPR RNA-guided FokI nucleases for efficient genome editing CRISPR
Publication (2014)CasOT: a genome-wide Cas9/gRNA off-target searching tool CRISPR
Publication (2014)Classification and evolution of type II CRISPR-Cas systems CRISPR
Publication (2014)Development and applications of CRISPR-Cas9 for genome engineering CRISPR
Publication (2014)Cut site selection by the two nuclease domains of the Cas9 RNA-guided endonuclease CRISPR
Publication (2014)Synthetic TAL effectors for targeted enhancement of transgene expression in plants TALEN
Publication (2013)Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes CRISPR
Publication (2013)Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and Deletions CRISPR
Publication (2013)Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans CRISPR
Publication (2013)Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas CRISPR
Publication (2013)The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori CRISPR
Publication (2013)Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila CRISPR
Publication (2013)Efficient genome editing in zebrafish using a CRISPR-Cas system CRISPR
Publication (2013)Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish CRISPR
Publication (2013)Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system CRISPR
Publication (2013)Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System CRISPR
Publication (2013)Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos CRISPR
Publication (2013)Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis CRISPR
Publication (2013)Effective gene targeting in rabbits using RNA-guided Cas9 nucleases CRISPR
Publication (2013)Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems CRISPR
Publication (2013)Heritable gene targeting in the mouse and rat using a CRISPR-Cas system CRISPR
Publication (2013)Generation of gene-modified mice via Cas9/RNA-mediated gene targeting CRISPR
Publication (2013)Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins CRISPR
Publication (2013)High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity CRISPR
Publication (2013)Phylogeny of Cas9 determines functional exchange ability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems CRISPR
Publication (2013)Genotyping with CRISPR-Cas-derived RNA-guided endonucleases CRISPR
Publication (2013)crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus CRISPR
Publication (2013)Programmable DNA cleavage in vitro by Cas9 CRISPR
Publication (2013)Genome engineering in cerevisiae using CRISPR-Cas systems CRISPR
Publication (2013)RNA-guided editing of bacterial genomes using CRISPR-Cas systems CRISPR
Publication (2013)Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system CRISPR
Publication (2013)Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 CRISPR
Publication (2013)Targeted genome modification of crop plants using a CRISPR-Cas system CRISPR
Publication (2013)Heritable genome editing in C. elegans via a CRISPR-Cas9 system CRISPR
Publication (2013)CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans CRISPR
Publication (2013)Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR–Cas9 System CRISPR
Publication (2013)Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease CRISPR
Publication (2013)Multiplex Genome Engineering Using CRISPR/Cas Systems CRISPR
Publication (2013)RNA-programmed genome editing in human cells CRISPR
Publication (2013)Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease CRISPR
Publication (2013)CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering CRISPR
Publication (2013)High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells CRISPR
Publication (2013)DNA targeting specificity of RNA-guided Cas9 nucleases CRISPR
Publication (2013)Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus CRISPR
Publication (2013)CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity CRISPR
Publication (2013)Orthogonal Cas9 proteins for RNA-guided gene regulation and editing CRISPR
Publication (2013)Efficient generation of large-scale genome-modified mice using gRNA and Cas9 endonuclease CRISPR
Publication (2013)Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity CRISPR
Publication (2013)CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes CRISPR
Publication (2013)Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice CRISPR
Publication (2013)Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA CRISPR
Publication (2013)Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis CRISPR
Publication (2013)The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems CRISPR
Publication (2013)High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.  TALEN
Publication (2012)A CRISPR/Cas system mediates bacterial innate immune evasion and virulence CRISPR
Publication (2012)Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria CRISPR
Publication (2012)RNA-Guided Human Genome Engineering via Cas9 CRISPR
Publication (2012)A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity CRISPR
Publication (2012)Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joining CRISPR
Publication (2011)The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli CRISPR
Publication (2011)CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III CRISPR
Publication (2011)A TALE nuclease architecture for efficient genome editing  TALEN
Publication (2011)Assembly of custom TALE-type DNA binding domains by modular cloning TALEN
Publication (2011)Genetic engineering of human pluripotent cells using TALE nucleases TALEN
Publication (2011)Transcriptional Activators of Human Genes with Programmable DNA-Specificity TALEN
Publication (2011)Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting TALEN
Publication (2011)Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription TALEN
Publication (2009)Short motif sequences determine the targets of prokaryotic CRISPR defence system CRISPR
Publication (2009)Breaking the code of DNA binding specificity of TAL-type III effectors TALEN
Publication (2009)A simple cipher governs DNA recognition by TAL effectors TALEN
Publication (2007)CRISPR provides acquired resistance against viruses in prokaryotes CRISPR
Publication (2005)Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin CRISPR
TrainingGene editing in pluripotent stem cellsCRISPR
VideoGenome editing video collectionCRISPR
WebinarGenome editing webinar collectionCRISPR, TALEN

仅供科研使用,不可用于诊断目的。