Precision and control for qPCR results you can trust

Applied Biosystems TaqMan and SYBR Green master mixes are designed to accurately and efficiently support your real-time PCR (qPCR) experiments. Optimized for improved data precision, carryover contamination control, extended benchtop stability, multiplexing, broad instrument compatibility, and superior performance, our real-time PCR master mixes enable worry-free runs and high-quality data.

Master mixes for increased workflow efficiency

Our pre-mixed, ready-to-use master mixes for qPCR are designed to increase laboratory productivity, save time, and ensure reproducibility. These formulations have been optimized and verified to ensure performance in a range of qPCR applications. Our master mixes contain buffer, dNTPs, passive reference dye, thermostable hot-start DNA polymerase, and other components formulated for reliable qPCR—no need to pipette low volumes of individual components. Simply add your sample and assay of choice and start your reactions.

TaqMan Fast master mixes have been optimized to deliver fast results even in the presence of common inhibitors, saving valuable instrument time and moving you to your next step sooner. These master mixes are also optimized for specific applications. For example, TaqMan Fast Virus 1-Step Master Mix is a 4X formulation that allows detection of very low titers of either DNA or RNA in fast reactions. This high concentration master mix allows more sample to be added, increasing the probability of detection even in a fast reaction.

Our SYBR Green master mixes deliver optimal specificity and reproducibility, just as you'd expect from the originators of Applied Biosystems™ SYBR™ Green dye. We continue to innovate to bring even better perforamce. For instance, PowerTrack SYBR Green Master Mix was formulated for a broad primer Tm and concentration compatibility, allowing flexible qPCR reaction setup with minimal optimization. The reagent also has built-in visual indicators to rapidly spot wells or tubes missing sample to prevent mistakes and wasted reagents.

ROX dye for improved data precision

ROX dye is an inert reference dye that improves the precision of results by normalizing spurious fluorescent signals arising from bubbles, evaporation, and other non-PCR-related artifacts. Applied Biosystems master mixes contain proprietary ROX dye specifically formulated for a wide range of real-time PCR instruments and compatible with most instrument light sources and filter sets.

 

ROX dye normalization helps qPCR users distinguish between PCR-related and non-PCR-related aberrations for easy experiment troubleshooting. With tightened standard deviations and increased quality control, you can have higher confidence in your results while running fewer replicates.

UNG/UDG for carryover contamination control

Preventing contamination is essential; even small amounts of carryover contamination can cause false positives. Uracil-N-glycosylase (UNG) and uracil-DNA glycosylase (UDG) are used to specifically degrade previous PCR amplifications or mis-primed, nonspecific products, leaving native nucleic acid templates intended for amplification intact. Choose TaqMan and SYBR Green master mixes with UNG or UDG for worry-free contamination control.

 

While UNG can be helpful in the reduction of contamination in many qPCR assays, it is not recommended for all applications including 1-step RT-PCR. In this application, the reverse transcription step to create cDNA would incorporate dU-nucleotides degraded by UNG in the reaction. Instead, UNG can be used in the second step of a two-step RT-PCR.

Extended benchtop stability

TaqMan Fast Advanced Master Mix has been engineered to retain its high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput liquid handling systems the confidence that the results on the first plate will mimic those on the last plate. For those with less extreme throughput needs, the enhanced stability of this master mix provides an added convenience to your workflow, as you are no longer constrained to immediately run your plates upon assembly.

Reaction changes over multiple cycles (A) at 0 hours and (B) after 72 hours show similar results, demonstrating exceptional stability.

Optimized for multiplexing

Get more data out of your samples without compromising sensitivity and accuracy. TaqMan multiplex real-time PCR solutions help you preserve sample material and minimize time-to-results while obtaining the same sensitivity as single-plex reactions.

Multiplexing is achieved by using probes conjugated to different fluorescent dyes corresponding to each target and requires careful design for optimal results. To amplify up to four targets in a single reaction, choose TaqMan Multiplex Master Mix. This mix is formulated to allow all amplifications to proceed despite the competition between reactions and is validated for quadplex gene expression and genotyping assays.

 

For added confidence in the results you see in every well, TaqMan Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). It is optimized for multiplexing with exogenous or endogenous control assays.

PCR efficiency

Results are shown for ß-actin (gene name ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions. The duplex reactions include the single-target ACTB and either a constant quantity of exogenous target (Figure A) or a relative quantity of endogenous target (Figure B).

Superior performance

TaqMan Fast Advanced Master Mix demonstrates unparalleled dynamic range

Applied Biosystems master mixes are designed to deliver high sensitivity, accuracy, dynamic range, and specificity. Our master mixes have been compared to those of other suppliers to ensure superior performance in every experiment.

 

All components within these master mixes—including buffer, dNTPs, passive reference dye, and thermostable hot-start DNA polymerase—are optimized for consistent results across a variety of applications and qPCR instruments.

 

Our scientists continuously seek new ways to improve our master mixes from thermal stable DNA polymerases to dyes that allow you to quickly spot a missed sample. The data below compare the dynamic range of TaqMan Fast Advanced Master Mix and the target specificity of PowerTrack SYBR Green Master Mix to other suppliers.

Dynamic range comparison between TaqMan Fast Advanced Master Mix and leading competitor products. Comparison of detection range (in number of logs) across a panel of various Applied Biosystems TaqMan Gene Expression Assays and TaqMan Multiplex Master Mix. The range of detection must have PCR efficiency between 85% and 115% and R2 values ≥0.98. Each master mix was tested using cDNA template and run according to manufacturers’ respective recommended protocols. Reactions (6 replicates) were run on the Applied Biosystems 7900HT Fast Real-Time PCR System.

Target specificity

Melt curve analyses for PGK1 (phosphoglyerate kinase 1), ARL1 (ADP-ribosylation factor-like protein 1), and SNF8 (vacuolar-sorting protein) targets were run on the QuantStudio 6 Flex Real-Time PCR System with nine different master mixes including PowerTrack SYBR Green Master Mix. Manufacturer’s recommended cycling conditions were used with each mix on a 6-log dilution series of human universal reference cDNA. UHR cDNA was run with assays PGK1, ARL1, and SNF8. 10-µL reactions were run in quadruplicate on the QuantStudio 6 Flex real-time PCR instrument running a 384-well block. Several competitors show amplification of non-specific product attributed to second peak in melt curve analysis.

 

Competitor Q1: Qiagen QuantiNova SYBR® Green PCR Kit; Competitor B: Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix; Competitor S: BioLine (Meridian Bioscience) SensiFAST™ SYBR® Lo-ROX Kit; Competitor Q2: Qiagen QuantiFast SYBR Green PCR Kit; Competitor QB: Quanta Biosciences PerfeCTa® SYBR® Green FastMix® Low ROX Reaction Mix; Competitor K: KAPA SYBR FAST qPCR kits, Low ROX; Competitor T: Takara TB Green® Premix Ex Taq™ II; Competitor P: ProMega GoTaq® qPCR Master Mix.

For Research Use Only. Not for use in diagnostic procedures.