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赛默飞世尔科技中国官网
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技术参考资源库
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The Molecular Probes Handbook
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第 1 章—荧光团及其胺基反应衍生物
Fluorophores and Their Amine-Reactive Derivatives—Chapter 1
展开导航
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Introduction to Amine Modification—Section 1.1
Kits for Labeling Proteins and Nucleic Acids—Section 1.2
Alexa Fluor Dyes Spanning the Visible and Infrared Spectrum—Section 1.3
BODIPY Dye Series—Section 1.4
Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
Coumarins, Pyrenes and Other Ultraviolet Light–Excitable Fluorophores—Section 1.7
Reagents for Analysis of Low Molecular Weight Amines—Section 1.8
Molecular Probes Handbook
Table of Contents
Fluorescence Fundamentals
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Chapter 2—Thiol-Reactive Probes
Chapter 3—Click Chemistry and other Functional Group Modifications
Chapter 4—Biotin and Hapten Derivatives
Chapter 5—Crosslinking and Photoactivatable Reagents
Chapter 6—Ultrasensitive Detection Technology
Chapter 7—Antibodies, Avidins and Lectins
Chapter 8—Nucleic Acid Detection and Analysis
Chapter 9—Protein Detection and Analysis
Chapter 10—Enzyme Substrates and Assays
Chapter 11—Probes for Cytoskeletal Proteins
Chapter 12—Probes for Organelles
Chapter 13—Probes for Lipids and Membranes
Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow
Chapter 15—Assays for Cell Viability, Proliferation and Function
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 17—Probes for Signal Transduction
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20—pH Indicators
Chapter 21—Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22—Probes for Membrane Potential
Chapter 23—Antifades and Other Tools for Fluorescence Applications
List of Tables
Technical Notes and Product Highlights
Introduction to Amine Modification—Section 1.1
Common Applications for Amine-Reactive Probes
Reactivity of Amino Groups
Isothiocyanates
Active Esters and Carboxylic Acids
Sulfonyl Chlorides
Other Amine-Reactive Reagents
Kits for Labeling Proteins and Nucleic Acids—Section 1.2
Kits for Labeling Proteins with a Fluorescent Dye
Kits for Labeling Proteins with Biotin or Dinitrophenyl (DNP)
Kits for Labeling Nucleic Acids with a Fluorescent Dye
Biotin Quantitation Assay Kits
Alexa Fluor Dyes Spanning the Visible and Infrared Spectrum—Section 1.3
Overview of the Alexa Fluor Dyes
Green-Fluorescent Alexa Fluor Dyes
Yellow-to-Red–Fluorescent Alexa Fluor Dyes
Far-Red– and Near-Infrared–Fluorescent Alexa Fluor Dyes
Blue-Fluorescent Alexa Fluor Dyes
Alexa Fluor Protein and Nucleic Acid Labeling Kits
Alexa Fluor Tandem Conjugates and Other Bioconjugates
Signal Amplification with Alexa Fluor Dyes
BODIPY Dye Series—Section 1.4
Overview of the BODIPY Fluorophores
Amine-Reactive BODIPY Dyes
BODIPY Dye Conjugates and Their Applications
Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
Fluorescein
Oregon Green 488 and Oregon Green 514 Dyes
Rhodamine Green and Rhodamine Green-X Dyes
Eosin
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Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
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Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
Tetramethylrhodamine
Lissamine Rhodamine B and Rhodamine Red-X Dyes
X-Rhodamine
Texas Red and Texas Red-X Dyes
Naphthofluorescein
Carboxyrhodamine 6G
QSY Dyes: Fluorescence Quenchers
Nonfluorescent Malachite Green
Coumarins, Pyrenes and Other Ultraviolet Light-Excitable Fluorophores—Section 1.7
Alexa Fluor 350 and Other Coumarin Derivatives
Pacific Orange Dye
Cascade Blue and Other Pyrene Derivatives
Cascade Yellow and Other Pyridyloxazole Derivatives
Naphthalenes, Including Dansyl Chloride
Dapoxyl Dye
Bimane
Reagents for Analysis of Low Molecular Weight Amines—Section 1.8
Fluorescamine
Dialdehydes: OPA and NDA
ATTO-TAG Reagents
7-Nitrobenz-2-Oxa-1,3-Diazole (NBD) Derivatives
Dansyl Chloride and Other Sulfonyl Chlorides
FITC and Other Isothiocyanates
Succinimidyl Esters and Carboxylic Acids
Related Tables
Molecular Probes amine-reactive dyes—Table 1.1
Active esters and kits for labeling proteins and nucleic acids—Table 1.2
Molecular Probes kits for protein and nucleic acid labeling—Table 1.3
Alexa Fluor active esters and kits for labeling proteins and nucleic acids—Table 1.4
Fluorescence quantum yields (QY) and lifetimes (τ) for Alexa Fluor dyes—Table 1.5
R<0> values for some Alexa Fluor dyes—Table 1.6
Amine-reactive BODIPY dyes—Table 1.7
Amine-reactive xanthene derivatives—Table 1.8
Amine-reactive, orange- and red-fluorescent fluorophores—Table 1.9
Molecular Probes nonfluorescent quenchers and photosensitizers—Table 1.10
R<0> values for QSY and dabcyl quenchers—Table 1.11
Amine-reactive, ultraviolet light-excitable fluorophores for labeling proteins and nucleic acids—Table 1.12
Amine-reactive, environment-sensitive fluorophores—Table 1.13
Technical Notes
The Alexa Fluor Dye Series—Note 1.1
Fluorescence Resonance Energy Transfer (FRET)—Note 1.2
Fluorescence Correlation Spectroscopy (FCS)—Note 1.3
Fluorescence Polarization (FP)—Note 1.4
Fluorescent Probes for Two-Photon Microscopy—Note 1.5
仅供科研使用,不可用于诊断目的。