| Dye | Abs * | Extinction Coefficient † | Amine-Reactive Dyes ‡ | Notes |
|---|
| Dabcyl | 453 | 32,000 | D2245 (SE) | - Broad and intense visible-wavelength absorption
- Efficient energy transfer acceptor from blue- and green-fluorescent dyes in FRET applications
|
| Malachite green | 628 | 76,000 | M689 (ITC) | - Nonfluorescent photosensitizer
|
| QSY 7 | 560 | 90,000 | Q10193 (SE) | - Essentially nonfluorescent quencher
- Broad visible-wavelength absorption
- Efficient energy transfer acceptor from UV light–excited green- and orange-fluorescent dyes in FRET applications
|
| QSY 9 | 562 | 88,000 | Q20131 (SE) | - Essentially nonfluorescent quencher
- Spectrally similar to QSY 7, but with enhanced water solubility
- Efficient energy transfer acceptor from UV light–excited green- and orange-fluorescent dyes in FRET applications
|
| QSY 21 | 661 | 90,000 | Q20132 (SE) | - Essentially nonfluorescent quencher
- Long-wavelength absorption
- Efficient energy transfer acceptor from red- and near-infrared–fluorescent dyes in FRET applications
|
| QSY 35 | 475 | 23,000 | Q20133 (SE) § | - Nonfluorescent quencher
- Spectrally similar to dabcyl
- Efficient energy transfer acceptor from blue- and green-fluorescent dyes in FRET applications
|
| * Absorption (Abs) maxima, in nm. † Molar extinction coefficient in cm-1M-1 determined at the wavelength listed in the column headed Abs. These values may vary with the environment, particularly for the QSY 35 dye. ‡ (ITC) = Isothiocyanate. (SE) = Succinimidyl ester. |