Flow Cytometry Protocols

• Super Bright Staining Buffer protocol
• Cell Preparation for Flow Cytometry Protocols (Invitrogen eBioscience reagents)
• Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers (Invitrogen eBioscience reagents)
• Staining Cell Surface Targets for Flow Cytometry (Invitrogen eBioscience reagents)
• Staining Intracellular Antigens for Flow Cytometry (Invitrogen eBioscience reagents)
• OneComp and UltraComp Compensation Beads Protocols for Flow Cytometry (Invitrogen eBioscience reagents)

• Isolation of human peripheral blood mononuclear cells from whole blood
• Monocyte isolation from peripheral blood mononuclear cells
• NK cell isolation from peripheral blood mononuclear cells
• T cell isolation from peripheral blood mononuclear cells
• CD4⁺ T cell isolation from peripheral blood mononuclear cells

• CD8⁺ T cell isolation from peripheral blood mononuclear cells
• Regulatory T cell isolation from peripheral blood mononuclear cells
• T cell isolation from secondary lymphoid organs
• Immune cell counting using automated cell counters
• Mouse Bone Marrow Cell Isolation
• Mouse Spleen Cell Isolation Protocol
• Whole Blood Staining Protocol for Flow Cytometry Analysis

• In vitro differentiation of macrophages from monocytes via M-CSF
• T-cell activation via functional antibodies anti-CD3 and anti-CD28
• Immune cell activation for cytokine production via LPS

• Stimulation of cytokine production in immune cells
• T-cell activation via Concanavalin A
• T-cell activation with Dynabeads Human T-Activator CD3/CD28

• LIVE/DEAD Fixable Dead Cell Stains Protocol
  
• LIVE/DEAD Sperm Viability Kit Flow Cytometry Protocol
• SYTOX Dead Cell Stains Protocol
• Viability Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)

• LIVE/DEAD Violet Viability/Vitality Kit Protocol
• Annexin V Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
• Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents)

• CellTrace™ CFSE Cell Proliferation Kit Protocol
• CellTrace™ Far Red Cell Proliferation Kit Protocol
• CellTrace™ Violet Cell Proliferation Kit Protocol
• BrdU and EdU Double Staining Protocol
• Click-iT EdU Protocol for Flow Cytometry
• Click-iT Plus EdU Flow Cytometry Assay Kits
• BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
• Staining Cells with eFluor Proliferation Dyes for Flow Cytometry (Invitrogen eBioscience reagents)

• Vybrant DyeCycle Violet Stain
• Vybrant DyeCycle Green and Orange Stains
• Vybrant DyeCycle Ruby Stain
• FxCycle Violet Stain
• FxCycle Violet Ready Flow Reagent
• FxCycle PI/RNase Staining Solution
• FxCycle Far Red Stain
• CellEvent Senescence Green Flow Cytometry Assay Kit

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