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产品信息
PHC9211
应用
建议稀释比
已发表文章
产品规格
种属
Human
Expression System
HEK293 cells
分子量
42 kDa
分类
Recombinant
类型
Protein
纯度
≥95% by SDS-PAGE and HPLC
内毒素浓度
<0.1 ng/µg
活性
ED50 ≤ 40 ng/mL; determined by the binding of immobilized human LRP6/Fc chimera receptro (3 µg/mL) to human Dkk-1 in a functional ELISA.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 0.1-0.5 mg/mL. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage: Store the lyophilized protein at 2-8°C or -20°C long term, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
靶标信息
DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: dickkopf 1 homolog; dickkopf related protein-1; Dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; Dickkopf-related protein 1; Dickkopf1; hDkk-1; hDkk1; SK
基因别名: DKK-1; DKK1; SK; UNQ492/PRO1008
UniProt ID: (Human) O94907
Entrez Gene ID: (Human) 22943
