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Invitrogen
Human Glucocorticoid Receptor Recombinant Protein
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Product Details
RP-500
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Expression System
Insect cells
Class
Recombinant
Type
Protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
RP-500A contains 50 µL of recombinant human glucocorticoid receptor at 3.5 mg/mL. RP-500B contains 20 µL of oligonucleotide at 100 ng/µL.
Each RP-500 is sufficient for 50 gel shift reactions or 50 lanes for Western blot detection with any ABR polyclonal Anti-human Glucocorticoid Receptor Antibody (PA1-510A, PA1-511A, PA1-512). Gel shift and Western blot protocols are supplied with RP-500 or available upon request. The components of this kit may be used to perform gel shift assays and with PA1-510A, Anti-Glucocorticoid Receptor antibody, gel super shift assays. This complete system can be used as a control system into which the researcher may introduce other components such as other putative response elements or other glucocorticoid receptors (mutants, from various tissue sources, etc.). The recombinant protein may also be used to perform DNase I footprinting experiments, as a control in Western blot experiments or for in vitro transcription assays.
RP-500A is recombinant human glucocorticoid receptor (full length) that was expressed in an insect cell expression system and enriched by ammonium sulfate precipitation followed by dialysis in KED buffer with 20% glycerol.
RP-500B is an oligonucleotide double stranded DNA representing the human tyrosine amino transferase gene response element with the following sequences: 5'...CTA GGC TGT ACA GGA TGT TCT GCC TAG...3'
3'...GAT CCG ACA TGT CCT ACA AGA CGG ATC...5'
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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