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Invitrogen
Mouse Fc gamma RI (CD64) His-tag Recombinant Protein
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产品信息
A42617
产品规格
种属
Mouse
Expression System
HEK293 cells
氨基酸序列
Mouse FCGR1, amino acids Met1-Pro297 (Accession # NP_034316.1) with a C-terminal His-tag
标签
His-tag
分子量
32 kDa
分类
Recombinant
类型
Protein
纯度
>95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC
内毒素浓度
<1.0 EU/µg
活性
1.Immobilized Rituximab (IgG1) at 1 µg/mL can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein, the EC50 is 16-35 ng/mL.
2.Immobilized Anti-CD3 antibody, mouse IgG2a at 2 µg/mL (100 µL/well) can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein, the EC50 is 0.8-2.4 ng/mL(QC tested).
3.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
4.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 0.28 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
5.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
6.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.69 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
7.The purity of Mouse Fc gamma RI (CD64) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
8.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein with an affinity constant of 3.46 nM as determined in BLI assay (Sartorius Octet RED384)(QC tested).
2.Immobilized Anti-CD3 antibody, mouse IgG2a at 2 µg/mL (100 µL/well) can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein, the EC50 is 0.8-2.4 ng/mL(QC tested).
3.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
4.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 0.28 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
5.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
6.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.69 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
7.The purity of Mouse Fc gamma RI (CD64) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
8.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein with an affinity constant of 3.46 nM as determined in BLI assay (Sartorius Octet RED384)(QC tested).
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
内含物
no preservative
保存条件
-20°C or -80°C if preferred
运输条件
Ambient
产品详细信息
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
靶标信息
CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD64; CD64 antigen; Fc-gamma receptor 1; Fc-gamma RI; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; IgG high affinity Fc receptor; RP11-196G18.2
基因别名: AI323638; AV092959; CD64; Fcg1; FcgammaRI; Fcgr1; IGGHAFC
UniProt ID: (Mouse) P26151
Entrez Gene ID: (Mouse) 14129
