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Invitrogen
Epinephrine/Adrenaline Competitive ELISA Kit
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产品规格
检测灵敏度
18.75 pg/mL
测定范围
31.25-2,000 pg/mL
检测样本类型/体积
{Cell Lysate=50 µL, Plasma=50 µL, Tissue Homogenate=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Adrenaline
均相（无需洗涤）
All
Species (reported)
All
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Epinephrine/Adrenaline (EPI) ELISA quantitates EPI in serum, plasma and other biological fluids.
原理方法
The Competitive multispecies ELISA research use only kit is designed to quantitatively measure The Epinephrine/Adrenaline (EPI) independent of species. A Epinephrine/Adrenaline standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve.Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Epinephrine/Adrenaline is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Epinephrine/Adrenaline in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Adrenaline, also known as epinephrine, plays a critical role in the body's acute stress response and is synthesized from noradrenaline in catecholaminergic neurons. The biosynthesis involves four key enzymes. The process begins with tyrosine conversion into L-DOPA by tyrosine hydroxylase, followed by L-DOPA conversion to dopamine by aromatic L-amino acid decarboxylase. Dopamine is subsequently converted into noradrenaline by dopamine-beta-hydroxylase. The final step is the conversion of noradrenaline into adrenaline through phenylethanolamine N-methyltransferase (PNMT), which utilizes the cofactor S-adenosyl-L-methionine to catalyze the methylation. Adrenaline exerts its physiological effects by binding to adrenergic receptors, which belong to the G protein-coupled receptor (GPCR) superfamily. These receptors are integral to mediating the cardiovascular effects of catecholamines, contributing to increased heart rate, blood pressure, and energy mobilization. The presence of adrenaline in the central nervous system (CNS) accounts for a small portion of catecholamines, yet its function is significant in synaptic transmission and neuromodulation, as it facilitates synchronized acetylcholine release, thus impacting neuronal communication and muscle function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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