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Invitrogen
Human Clusterin ELISA Kit
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产品规格
检测灵敏度
15 pg/mL
测定范围
15-20,000 pg/mL
检测样本类型/体积
{Plasma=1 µL, Serum=1 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
Apolipoprotein J
蛋白家族
Mitochondrial Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Clusterin ELISA quantitates Hu Clusterin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Clusterin.
原理方法
The Human Clusterin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : AAG4, APO-J, APOJ, CLI, CLU, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2
基因ID : (Human) 1191
基因符号 : CLU
蛋白别名 : Aging associated protein 4, apo j, Apo J a, Apo J alpha, Apo J b, Apo J beta, Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B), Clusterin alpha / beta chain, Complement associated protein SP40, GP80, HGNC:2095, Ku70-binding protein 1 (KUB1), MGC24903, ApoJ b, ApoJ beta, ApoJ α, ApoJ β, ApoJa, ApoJb, Apo J α, Apo J β, Apo Ja, Apo Jb, ApoJ a, ApoJ alpha, Aging-associated gene 4 protein, aging-associated protein 4, Apo-J, Apolipoprotein J, CLI, Clusterin, Complement cytolysis inhibitor, complement lysis inhibitor, Complement-associated protein SP-40, 40, Ku70-binding protein 1, NA1/NA2, SGP-2, Sulfated glycoprotein 2, Testosterone-repressed prostate message 2, TRPM-2
UniProt ID (Human) P10909
