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Invitrogen
Human Endothelin-1 (ET-1) ELISA Kit
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产品规格
检测灵敏度
0.579 pg/mL
测定范围
0.781-100 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=2.5 µL, Urine=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
3.7%
批内差异
3.3%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Extraction Solution
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Extraction Solution
运输条件
Wet ice
储存
-20°C
蛋白名称
Endothelin 1
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Endothelin-1 (ET-1) Multispecies ELISA quantitates Endothelin-1 in human, bovine, procine, dog, rat, and mouse serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Endothelin-1.
原理方法
The Endothelin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME
基因ID : (Human) 1906
基因符号 : EDN1
蛋白别名 : Endothelin-1, PPET1, Preproendothelin-1, preproendothelin-1, Big endothelin 1, Big ET, Big ET 1, Big ET1, BigET, Et
UniProt ID (Human) P05305
