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Invitrogen
Human Ubiquitin ELISA Kit
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产品规格
检测灵敏度
30 pg/mL
测定范围
30-20,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
Ubiquitin
蛋白家族
Other Proteins
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Ubiquitin 1 RPS27A ELISA quantitates Hu Ubiquitin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Ubiquitin.
原理方法
The Human Ubiquitin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CEP52, CEP80, HEL-S-50, HEL112, HMG20, HUBCEP52, L40, RPL40, RPS27A, RPS27L, S27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, UBCEP80
基因符号 : RPS27A, RPS27L, UBA52, UBB, UBC
蛋白别名 : 40S ribosomal protein S27-like, 40S ribosomal protein S27a, CEP52, epididymis luminal protein 112, epididymis secretory protein Li 50, polyubiquitin B, Polyubiquitin-B, Polyubiquitin-C, Ribosomal protein eS27-like, ribosomal protein S27-like, RPS27L, Small ribosomal subunit protein eS27-like, Ubiquitin A-52 residue ribosomal protein fusion product 1, ubiquitin and ribosomal protein S27a, ubiquitin C, ubiquitin carboxyl extension protein 52, Ubiquitin carboxyl extension protein 80, Chap1, hPLIC-2, HRIHFB2157, HUBCEP52, OTTHUMP00000200786, UBC, Ubiquilin 2, OTTHUMP00000201692, PLIC-2, RPL40, RPS27A, UBA52, UBB, ubiquitin-52 amino acid fusion protein, ubiquitin-CEP52, ubiquitin-CEP80, Ubiquitin-ribosomal protein eL40 fusion protein, Ubiquitin-ribosomal protein eS31 fusion protein
UniProt ID (Human) P62979, (Human) Q71UM5, (Human) P62987, (Human) P0CG47, (Human) P0CG48
