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Invitrogen
Mouse Clusterin Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.19 ng/mL
Assay range
0.31-20 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Apolipoprotein J
Species (tested)
Mouse
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Mouse CLU (Clusterin) ELISA quantitates CLU in serum, plasma and other biological fluids.
Principle of the method
The Mouse Clusterin (CLU) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Clusterin (CLU) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Clusterin (CLU). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Clusterin (CLU) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AI893575, ApoJ, Cli, Clu, D14Ucla3, Msgp-2, Sgp-2, Sgp2, SP-40, Sugp-2
Gene ID : (Mouse) 12759
Gene symbol : Clu
Protein Aliases : Aging associated protein 4, apo j, Apo J a, Apo J alpha, Apo J b, Apo J beta, Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B), Clusterin alpha / beta chain, Complement associated protein SP40, GP80, HGNC:2095, Ku70-binding protein 1 (KUB1), MGC24903, ApoJ b, ApoJ beta, ApoJ α, ApoJ β, ApoJa, ApoJb, Apo J α, Apo J β, Apo Ja, Apo Jb, ApoJ a, ApoJ alpha, Apo-J, Apolipoprotein J, Clusterin, Clustrin, complement lysis inhibitor, SGP-2, Sulfated glycoprotein 2, testosterone repressed prostate message, testosterone repressed prostate message-2
UniProt ID (Mouse) Q06890
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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