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Invitrogen
Rat CRP ELISA Kit
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产品规格
检测灵敏度
2 ng/mL
测定范围
2-133 ng/mL
检测样本类型/体积
{Plasma=1 µL, Serum=1 µL, Supernatant=50 µL, Urine=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated ELISA Plate
Detection Antibody
Standard
Wash Buffer Powder
Substrate Solution
Stop Solution
Detection Antibody
Standard
Wash Buffer Powder
Substrate Solution
Stop Solution
运输条件
Wet ice
储存
2-8°C
蛋白名称
CRP
Target site-specificity
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Rat C-Reactive Protein (CRP) ELISA quantitates Rt CRP in rat serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt CRP.
原理方法
The Rt CRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
