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Invitrogen
Vitamin D3 Competitive ELISA Kit
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产品规格
检测灵敏度
0.94 ng/mL
测定范围
1.56-100 ng/mL
检测样本类型/体积
{Cell Lysate=50 µL, Plasma=50 µL, Serum=50 µL, Tissue Homogenate=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Vitamin D3
均相（无需洗涤）
All
Species (reported)
All
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Vitamin D3 (VD3) ELISA quantitates VD3 in serum, plasma and other biological fluids.
原理方法
The competitive multispecies ELISA research kit is designed to quantitatively measure Vitamin D3, independent of species . A Vitamin D3 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Vitamin D3 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. A TMB substrate solution is then added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Vitamin D3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Vitamin D3, also known as cholecalciferol, is a form of vitamin D that is synthesized in the skin upon exposure to ultraviolet B (UVB) light from the sun. It is the most biologically active form of vitamin D and plays a crucial role in maintaining overall health. Structurally, vitamin D3 is a steroid hormone precursor that undergoes metabolic processes in the liver and kidneys to be converted into its active form, known as calcitriol. Calcitriol acts as a hormone that regulates calcium and phosphorus metabolism in the body. The main function of vitamin D3 is to facilitate the absorption of calcium and phosphorus from the intestine, promoting their utilization for bone health and growth. It aids in the regulation of bone mineralization, ensuring the proper formation and maintenance of strong and healthy bones. Vitamin D3 is also involved in various other physiological processes beyond bone health. It plays a vital role in modulating immune function, helping to regulate the immune response and reduce the risk of autoimmune diseases. Additionally, it has been linked to cardiovascular health, muscle function, and mood regulation. In terms of localization, vitamin D receptors are present in various tissues throughout the body, including the intestines, bones, kidneys, immune cells, and brain. This widespread distribution of vitamin D receptors highlights the importance of maintaining adequate vitamin D3 levels for optimal functioning of these tissues and overall health.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Cholecalciferol
