The Tango™ CHRM2-bla U2OS cells contain the human Cholinergic muscarinic 2 receptor (CHRM2) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ CHRM2-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of Carbachol. In addition, Tango™ CHRM2-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
For Research Use Only. Not for use in diagnostic procedures.
规格
编录号
NM_000739.2
检测条目
基于细胞的 β-arrestin 募集
细胞状态
分裂细胞
胞质尾区
来自 GRPR GPCR 的尾部
检测方法
荧光
基因符号
M2
种群状态
单细胞克隆起源
产品线
Tango™
报告基因
BLA(β-内酰胺酶)
运输条件
室温
目标条目
CHRM2, M2
细胞系
U2OS
System Type
Tango™
Druggable Target
GPCR
内容与储存
(LiveBLAzer™-FRET B/G 上样试剂盒、溶液 D 和其他材料应要求单独提供;请参阅方案)。 包括: o Tango™ CHRM2-bla U2OS 细胞 o Tango™ CHRM2-bla U2OS 测定方案 检验报告 Tango™ CHRM2-bla U2OS 细胞在干冰上运输,接收后应立即储存于液氮中。