Identification of the sex-determining locus of Atlantic salmon (Salmo salar) on chromosome 2.
AuthorsArtieri CG, Mitchell LA, Ng SH, Parisotto SE, Danzmann RG, Hoyheim B, Phillips RB, Morasch M, Koop BF, Davidson WS,
JournalCytogenet Genome Res
PubMed ID16276105
'We have integrated data from linkage mapping, physical mapping and karyotyping to gain a better understanding of the sex-determining locus, SEX, in Atlantic salmon (Salmo salar). SEX has been mapped to Atlantic salmon linkage group 1 (ASL1) and is associated with several microsatellite markers. We have used probes designed from ... More
Oligonucleotide microarray analysis of genomic imbalance in children with mental retardation.
AuthorsFriedman JM, Baross A, Delaney AD, Ally A, Arbour L, Armstrong L, Asano J, Bailey DK, Barber S, Birch P, Brown-John M, Cao M, Chan S, Charest DL, Farnoud N, Fernandes N, Flibotte S, Go A, Gibson WT, Holt RA, Jones SJ, Kennedy GC, Krzywinski M, Langlois S, Li HI, McGillivray BC, Nayar T, Pugh TJ, Rajcan-Separovic E, Schein JE, Schnerch A, Siddiqui A, Van Allen MI, Wilson G, Yong SL, Zahir F, Eydoux P, Marra MA,
JournalAm J Hum Genet
PubMed ID16909388
'The cause of mental retardation in one-third to one-half of all affected individuals is unknown. Microscopically detectable chromosomal abnormalities are the most frequently recognized cause, but gain or loss of chromosomal segments that are too small to be seen by conventional cytogenetic analysis has been found to be another important ... More
Beringian origins and cryptic speciation events in the fly agaric (Amanita muscaria).
AuthorsGeml J, Laursen GA, O'neill K, Nusbaum HC, Taylor DL,
JournalMol Ecol
PubMed ID16367842
'Amanita muscaria sensu lato has a wide geographic distribution, occurring in Europe, Asia, Africa, Australia, New Zealand, and North, Central and South America. Previous phylogenetic work by others indicates three geographic clades (i.e. ''Eurasian'', ''Eurasian-alpine'' and ''North American'' groups) within A. muscaria. However, the historical dispersal patterns of A. muscaria ... More
RNA interference of the clock gene period disrupts circadian rhythms in the cricket Gryllus bimaculatus.
AuthorsMoriyama Y, Sakamoto T, Karpova SG, Matsumoto A, Noji S, Tomioka K,
JournalJ Biol Rhythms
PubMed ID18663238
'Periodic expression of so-called clock genes is an essential part of the circadian clock. In Drosophila melanogaster the cyclic expression of per and tim through an autoregulatory feedback loop is believed to play a central role in circadian rhythm generation. However, it is still elusive whether this hypothesis is applicable ... More
PCR detection of staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk.
'Milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcus aureus. This study aimed to analyze the frequency of genes encoding the staphylococcal enterotoxins (SEs) SEA, SEB, SEC, SED, SEE, SEG, ... More
Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications.
AuthorsHuijsmans CJ, Damen J, van der Linden JC, Savelkoul PH, Hermans MH,
JournalBMC Res Notes
PubMed ID20840759
'A large portion of tissues stored worldwide for diagnostic purposes is formalin-fixed and paraffin-embedded (FFPE). These FFPE-archived tissues are an extremely valuable source for retrospective (genetic) studies. These include mutation screening in cancer-critical genes as well as pathogen detection. In this study we evaluated the impact of several widely used ... More
Characterization of fHbp, nhba (gna2132), nadA, porA, and sequence type in group B meningococcal case isolates collected in England and Wales during January 2008 and potential coverage of an investigational group B meningococcal vaccine.
AuthorsLucidarme J, Comanducci M, Findlow J, Gray SJ, Kaczmarski EB, Guiver M, Vallely PJ, Oster P, Pizza M, Bambini S, Muzzi A, Borrow R,
JournalClin Vaccine Immunol
PubMed ID20375242
'Invasive disease caused by meningococcal capsular groups A, C, W-135, and Y is now preventable by means of glycoconjugate vaccines that target their respective polysaccharide capsules. The capsule of group B meningococci (MenB) is poorly immunogenic and may induce autoimmunity. Vaccines based on the major immunodominant surface porin, PorA, are ... More
TTC staining of damaged brain areas after MCA occlusion in the rat does not constrict quantitative gene and protein analyses.
AuthorsKramer M, Dang J, Baertling F, Denecke B, Clarner T, Kirsch C, Beyer C, Kipp M,
JournalJ Neurosci Methods
PubMed ID20064557
'In models of ischemic stroke, TTC (2,3,5-triphenyltetrazolium chloride) staining is commonly applied for the fast and reliable visualization of hypoxic brain tissue and for defining the size of cerebral infarction and penumbra. Deciphering molecular processes of pathogenesis within the penumbra is of particular interest for the development of therapeutic strategies. ... More
Method for rapid detection of viable Escherichia coli in water using real-time NASBA.
AuthorsHeijnen L, Medema G,
JournalWater Res
PubMed ID19476965
'A rapid real-time NASBA method was developed for detection of Escherichia coli in water samples. In this method, a fragment of the clpB-mRNA is amplified and a specific molecular beacon probe is used to detect the amplified mRNA fragment during the NASBA reaction. The method was shown to be specific ... More
Mucor circinelloides was identified by molecular methods as a cause of primary cutaneous zygomycosis.
AuthorsIwen PC, Sigler L, Noel RK, Freifeld AG,
JournalJ Clin Microbiol
PubMed ID17122018
A case of primary cutaneous zygomycosis caused by Mucor circinelloides is described. Histopathology showed typical hyphae along with chlamydospores. The isolate was identified by molecular and phenotypic methods. The utility of sequence analysis of the internal transcribed spacer region is highlighted; however, further studies are needed to assess species genetic ... More
Simple, robust methods for high-throughput nanoliter-scale DNA sequencing.
AuthorsSmailus DE, Marziali A, Dextras P, Marra MA, Holt RA,
JournalGenome Res
PubMed ID16169928
We have developed high-throughput DNA sequencing methods that generate high quality data from reactions as small as 400 nL, providing an approximate order of magnitude reduction in reagent use relative to standard protocols. Sequencing of clones from plasmid, fosmid, and BAC libraries yielded read lengths (PHRED20 bases) of 765 +/- ... More
TLR9-targeted biodegradable nanoparticles as immunization vectors protect against West Nile encephalitis.
AuthorsDemento SL, Bonafé N, Cui W, Kaech SM, Caplan MJ, Fikrig E, Ledizet M, Fahmy TM,
JournalJ Immunol
PubMed ID20660705
Vaccines that activate humoral and cell-mediated immune responses are urgently needed for many infectious agents, including the flaviviruses dengue and West Nile (WN) virus. Vaccine development would be greatly facilitated by a new approach, in which nanoscale modules (Ag, adjuvant, and carrier) are assembled into units that are optimized for ... More
Novel urine hepcidin assay by mass spectrometry.
AuthorsKemna E, Tjalsma H, Laarakkers C, Nemeth E, Willems H, Swinkels D,
JournalBlood
PubMed ID16030189
The hepatic peptide hormone hepcidin is the central regulator of iron metabolism and mediator of anemia of inflammation. To date, only one specific immuno-dot assay to measure hepcidin in urine had been documented. Here we report an alternative approach for quantification of hepcidin in urine by surface-enhanced laser desorption/ionization time-of-flight ... More
Decrease in creatine kinase messenger RNA expression in the hippocampus and dorsolateral prefrontal cortex in bipolar disorder.
AuthorsMacDonald ML, Naydenov A, Chu M, Matzilevich D, Konradi C,
JournalBipolar Disord
PubMed ID16696827
Bipolar disorder (BPD) affects more than 2 million adults in the USA and ranks among the top 10 causes of worldwide disabilities. Despite its prevalence, very little is known about the etiology of BPD. Recent evidence suggests that cellular energy metabolism is disturbed in BPD. Mitochondrial function is altered, and ... More
Evaluation of methods used to concentrate and detect hepatitis A virus in water samples.
AuthorsVillar LM, de Paula VS, Diniz-Mendes L, Lampe E, Gaspar AM,
JournalJ Virol Methods
PubMed ID16887200
Two adsorption-elution concentration methods, both involving negatively charged membranes, were evaluated in order to monitor hepatitis A virus (HAV) contamination in tap, river, mineral and coastal water samples: elution with urea-arginine phosphate buffer/reconcentration with magnesium chloride (method 1); and sodium hydroxide elution/reconcentration with a commercial concentrator (method 2). Nested (qualitative) ... More
Selective regulation of growth factor expression in cultured cortical astrocytes by neuro-pathological toxins.
AuthorsBraun A, Dang J, Johann S, Beyer C, Kipp M,
JournalNeurochem Int
PubMed ID19524632
Astrocytes are integrated in the complex regulation of neurodegeneration and neuronal damage in the CNS. It is well-known that astroglia produces a plethora of growth factors which might be protective for neurons. Growth factors prevent neurons from cell death and promote proliferation and differentiation of precursor cells. Previous data suggest ... More
Differential modulation of beta-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines.
AuthorsDerache C, Esnault E, Bonsergent C, Le Vern Y, Quéré P, Lalmanach AC,
JournalDev Comp Immunol
PubMed ID19539093
beta-Defensins are important components of innate immunity in mucosal tissue, a major entry site for several pathogens. These small cationic peptides possess antimicrobial activity against various microorganisms including Salmonella. Two chicken inbred lines, 6 and 15I, diverge phenotypically with respect to levels of Salmonella Enteritidis intestinal carriage and to level ... More
Impairing Otp homeodomain function in oral ectoderm cells affects skeletogenesis in sea urchin embryos.
AuthorsCavalieri V, Spinelli G, Di Bernardo M,
JournalDev Biol
PubMed ID14512022
In the sea urchin embryo skeletogenesis is the result of a complex series of molecular and cellular events that coordinate the morphogenetic process. Past and recent evidence strongly indicate that skeletal initiation and growth are strictly dependent on signals emanating from the oral ectodermal wall. As previously suggested, Orthopedia (Otp), ... More
Apolipoprotein c1 association with hepatitis C virus.
AuthorsMeunier JC, Russell RS, Engle RE, Faulk KN, Purcell RH, Emerson SU,
JournalJ Virol
PubMed ID18667498
Accumulating evidence suggests that cellular lipoprotein components are involved in hepatitis C virus (HCV) morphogenesis, but the precise contribution of these components remains unclear. We investigated the involvement of apolipoprotein C1 (ApoC1) in HCV infection in the HCV pseudotyped particle system (HCVpp), in the recently developed cell culture infection model ... More
Genotyping of Clostridium perfringens isolated from calves with neonatal diarrhea.
AuthorsFerrarezi MC, Cardoso TC, Dutra IS,
JournalAnaerobe
PubMed ID19114113
Neonatal diarrhea is one of the main causes of losses in cattle herds. Clostridium perfringens is a widespread enteropathogen, and is responsible for many animal diseases such as bovine neonatal diarrhea. Fecal samples from 141 diarrheic calves and 129 healthy calves, aged up to 28 days and belonging to three ... More
One-step reverse transcriptase polymerase chain reaction for the diagnosis of respiratory syncytial virus in children.
Authorsdo Nascimento CA, Leal AL, Souza TS, de Moraes CT, Comone P, Tenório EC, Vedovello D, Quinzani RH, Gilio AE, Vieira SE, Durigon EL, Botosso VF, Sant'Anna OA,
JournalJ Virol Methods
PubMed ID18068812
Human respiratory syncytial virus (HRSV) is the main cause of acute lower respiratory tract infections in infants and children. Rapid diagnosis is required to permit appropriate care and treatment and to avoid unnecessary antibiotic use. Reverse transcriptase (RT-PCR) and indirect immunofluorescence assay (IFA) methods have been considered important tools for ... More