Brinster's, no Glutamine, no phenol red
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Gibco™

Brinster's, no Glutamine, no phenol red

Brinster's medium for ovum culture (BMOC) is a simple chemically defined media containing bovine serum albumin (BSA) developed by RalphRead more
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Catalog NumberQuantity
11126034100 mL
Catalog number 11126034
Price (CNY)
657.20
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Ends: 31-Dec-2025
839.00
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Quantity:
100 mL
Price (CNY)
657.20
Online Exclusive
Ends: 31-Dec-2025
839.00
Save 181.80 (22%)
Each
Add to cart

Brinster's medium for ovum culture (BMOC) is a simple chemically defined media containing bovine serum albumin (BSA) developed by Ralph Brinster for in vitro development of mouse oocytes. This medium is a variation of the original formulation as it does not contain antibiotics. Brinster's medium has also been used for culture of other mammalian embryos during the early stages of development.

This Brinster's medium is manufactured as follows:
WithWithout
• Bovine Serum Albumin• L-Glutamine
• Sodium pyruvate• HEPES
 • Phenol Red


The complete formulation is available.

Gibco™ Brinster's medium is unique from other media as it contains no amino acids or vitamins. Brinster's medium is supplemented with bovine serum albumin required for supporting oocyte maturation and the early stages of mammalian embryo development and pyruvate as an essential energy source.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Brinster's medium uses a sodium bicarbonate buffer system (2.1 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMouse Oocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeBrinster's Medium
Quantity100 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
SterilitySterile-filtered
With AdditivesLow Glucose, Sodium Pyruvate, Bovine Serum Albumin (BSA)
Without AdditivesNo Glutamine, No HEPES, No Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Brinster's, no Glutamine, no phenol red FAQs