Invitrogen™

BaculoDirect™ C 端表达试剂盒

货号: 12562013
Invitrogen™

BaculoDirect™ C 端表达试剂盒

货号: 12562013
货号
12562013
又称 12562-013
单位规格
5 transfections
价格(CNY)
货号单位规格价格(CNY)
12562013
又称 12562-013
5 transfections联系我们 ›
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The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway™ Technology with baculovirus expression makes the BaculoDirect System the fastest and easiest method for production of recombinant baculovirus.

How it works
BaculoDirect™ Linear DNA (the baculovirus genome) is engineered to include attR sites for quick and efficient recombination with a Gateway entry clone. The gene of interest is recombined from the entry clone into the BaculoDirect Linear DNA using a simple, one-hour LR reaction (Figure 1). The resulting reaction mix contains the recombinant baculovirus carrying the gene of interest and is used to transfect insect cells. The need for transforming bacteria and isolating a large bacmid or co-transfecting a transfer vector and linear baculovirus DNA into insect cells is eliminated. As a result, the hands-on time is greatly reduced. Purified baculovirus can be isolated in less than one week.

Gateway linear DNA
BaculoDirect Linear DNA is designed for rapid cloning with a Gateway entry clone and subsequent expression in Sf9 or Sf21 insect cells. The Linear DNA features:

• Strong polyhedrin promoter for high-level expression
• R-sites for efficient recombination with any attL-flanked Gateway entry vector
• TK gene for negative selection using ganciclovir
• C-terminal V5-His tag (BaculoDirect™ C-Term Expression Kit) or N-terminal V5-His tag (BaculoDirect™ N-Term Expression Kit) for detection with anti-V5 antibody and purification with nickel-chelating resin
• TEV protease cleavage site for removal of the V5-His tag following purification (BaculoDirect N-Term Expression Kit)
• LacZ gene, to ensure a pure baculovirus stock is generated, which is replaced by your gene of interest after LR recombinant reaction

Additional materials required, available separately: Gateway entry clone.

A selection guide for choosing the most appropriate Gateway entry vector for your application can be found at: www.thermofisher.cn/Gateway.

For Research Use Only. Not for use in diagnostic procedures.

规格

促进剂
多角体蛋白
产品类型
表达试剂盒
蛋白标记
His 标签 (6x)、V5 抗原决定簇标签, V5 Epitope Tag
克隆方法
Gateway™
载体
BaculoDirect 载体
产品线
BaculoDirect™、Gateway™, Gateway™

内容与储存

BaculoDirect 表达试剂盒包括 5 个小瓶(0.3 µg/瓶)的 BaculoDirect 线性 DNA、125 µL Cellfectin 转染试剂、冷冻 Sf9 细胞、Gateway LR Clonase 酶混合物、GIBCO 无添加剂的 Grace 昆虫培养基和更昔洛韦。

在 +4°C 下储存 BaculoDirect 线性 DNA、Cellfectin 试剂和 Grace 培养基。在 -80°C 下储存 LR Clonase 混合物。将 Sf9 细胞储存于液氮中。如果妥善储存,可保证所有组分稳定储存 6 个月。

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