Search
Search
View additional product information for Nunc™ Thermanox™ Coverslips - FAQs (174934, 174969, 174942, 174985, 174977, 150067, 174950)
9 product FAQs found
Thermanox Coverslips are auto-fluorescent within the 380nm - 545nm range.
Please review the following information regarding the dimensions of our Nunc Thermanox Coverslips:
Cat. No.; Dimensions:
- 174950; 13 mm diameter
- 174969; 15 mm diameter
- 174977; 22 mm diameter
- 174985; 25 mm diameter
- 150067; 24 mm x 30 mm
- 174934; 10.5 mm x 22 mm
- 174942; 22 mm x 60 mm
Our Nunc Thermanox Coverslips are 0.2 mm thickness.
The thickness grade ranges as follows:
- No. 0: 0.085-0.13 mm
- No. 1: 0.13-0.16 mm
- No. 1.5: 0.16-0.19 mm
- No. 2: 0.19-0.23 mm
Based upon the above thickness grade ranges, our Nunc Thermanox Coverslips have a No.2 thickness rating.
Our Nunc Thermanox Coverslips are 0.2 mm in thickness.
Yes, our Nunc Thermanox Coverslips can be autoclaved at 20 min steam, 20 min dry, at 120 degrees C.
Our Nunc Thermanox Coverslips autofluoresce at the rhodamine and FITC range.
Fluorescein isothiocyanate:
- excitation = 494 nm
- emission = 517 nm
A simple method, to determine which side of our Nunc Thermanox Coverslip is cell cultured-treated, is the "droplet" test. A drop of water or culture medium will spread on the hydrophilic, cell culture treated side.
Please review the following procedure for the "droplet" test:
- Put a drop of sterile media or water on the coverslip
- If the droplet forms a bead, then that side of the coverslip is not treated
- If the droplet spreads evenly over the coverslip, then that side of the coverslip is treated
Additionally, the coverslips are packaged so the cell culture-treated side faces the label.
Thermanox is a trademark name for a particular type of polymer in the polyolefin family.
If Thermanox coverslips are still in the original packaging, the side facing the label is treated. If they have been removed, there is another way to determine this: hold one with forceps at a slight angle to the horizontal and dispense a few drops of sterile water onto the surface. On the untreated side the water will bead up tightly as it runs off, but on the treated side it will spread out much more due to the hydrophilic surface modification.