Identification and characterization of RPK118, a novel sphingosine kinase-1-binding protein.
AuthorsHayashi S, Okada T, Igarashi N, Fujita T, Jahangeer S, Nakamura S
JournalJ Biol Chem
PubMed ID12077123
'Sphingosine kinase (SPHK) is a key enzyme catalyzing the formation of sphingosine 1 phosphate (SPP), a lipid messenger that is implicated in the regulation of a wide variety of important cellular events through intracellular as well as extracellular mechanisms. However, the molecular mechanism of the intracellular actions of SPP remains ... More
Modulation of mouse Paneth cell alpha-defensin secretion by mIKCa1, a Ca2+-activated, intermediate conductance potassium channel.
Authors Ayabe Tokiyoshi; Wulff Heike; Darmoul Dalila; Cahalan Michael D; Chandy K George; Ouellette Andre J;
JournalJ Biol Chem
PubMed ID11724775
'Paneth cells in small intestinal crypts secrete microbicidal alpha-defensins in response to bacteria and bacterial antigens (Ayabe, T., Satchell, D. P., Wilson, C. L., Parks, W. C., Selsted, M. E., and Ouellette, A. J. (2000) Nat. Immunol. 1, 113- 38). We now report that the Ca(2+)-activated K(+) channel mIKCa1 modulates ... More
The cathepsin B of Toxoplasma gondii, toxopain-1, is critical for parasite invasion and rhoptry protein processing.
Authors Que Xuchu; Ngo Huân; Lawton Jeffrey; Gray Mary; Liu Qing; Engel Juan; Brinen Linda; Ghosh Partho; Joiner Keith A; Reed Sharon L;
JournalJ Biol Chem
PubMed ID12000756
'Cysteine proteinases play a major role in invasion and intracellular survival of a number of pathogenic parasites. We cloned a single copy gene, tgcp1, from Toxoplasma gondii and refolded recombinant enzyme to yield active proteinase. Substrate specificity of the enzyme and homology modeling identified the proteinase as a cathepsin B. ... More
Misexpression of the eyes absent family triggers the apoptotic program.
Authors Clark S Wesley; Fee Brian E; Cleveland John L;
JournalJ Biol Chem
PubMed ID11700312
'Genetic studies in Drosophila and mice have shown that eyes absent (eya) is an important and conserved transcriptional regulator of development. Along with eyeless/Pax6, sine oculis, and dachshund, eya genes function as master regulators in eye development and can induce ectopic eye formation. Furthermore, the loss-of-function mutants of these genes ... More
A novel zinc finger transcription factor with two isoforms that are differentially repressed by estrogen receptor-alpha.
Authors Conroy Andrew T; Sharma Manju; Holtz Ann E; Wu Chengbiao; Sun Zijie; Weigel Ronald J;
JournalJ Biol Chem
PubMed ID11779858
'Estrogen receptor-alpha (ERalpha) can induce the expression of genes in response to estrogen by binding to estrogen response elements in the promoters of target genes. There is growing evidence that ERalpha can alter patterns of gene expression in response to ligand by regulating the activity of other factors through a ... More
Macrophage-restricted and Interferon gamma -inducible Expression of the Allograft Inflammatory Factor-1 Gene Requires Pu.1.
Authors Sibinga Nicholas E S; Feinberg Mark W; Yang Hongyuan; Werner Frank; Jain Mukesh K;
JournalJ Biol Chem
PubMed ID11861656
'Expression of allograft inflammatory factor-1 (Aif-1), a 17-kDa protein bearing an EF-hand Ca(2+) binding motif, increases markedly in monocytes and macrophages participating in allo- and autoimmune reactions, including the perivascular inflammation in transplanted hearts, microglial infiltrates in experimental autoimmune neuritis, and the inflamed pancreas of prediabetic BB rats. To investigate ... More
Cytokine-induced down-regulation of zfm1/splicing factor-1 promotes smooth muscle cell proliferation.
'One hallmark of inflammation is the proliferation of bystander cells such as vascular smooth muscle cells (SMC), a process governed by growth factors and cytokines. Whereas cytokine induction of gene products promoting inflammation and proliferation is well characterized, little is known about the concomitant down-regulation of potentially counter-regulatory gene products ... More
Molecular characterization of the starfish inositol 1,4,5-trisphosphate receptor and its role during oocyte maturation and fertilization.
The release of calcium ions (Ca(2+)) from their intracellular stores is essential for the fertilization of oocytes of various species. The calcium pools can be induced to release Ca(2+) via two main types of calcium channel receptor: the inositol 1,4,5-trisphosphate receptor (IP(3)R) and the ryanodine receptor. Starfish oocytes have often ... More
SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase localized to mitochondria.
The SIR2 (silent information regulator 2) gene family has diverse functions in yeast including gene silencing, DNA repair, cell-cycle progression, and chromosome fidelity in meiosis and aging. Human homologues, termed sirtuins, are highly conserved but are of unknown function. We previously identified a large imprinted gene domain on 11p15.5 and ... More
Arabidopsis COP10 is a ubiquitin-conjugating enzyme variant that acts together with COP1 and the COP9 signalosome in repressing photomorphogenesis.
A group of evolutionarily conserved pleiotropic COP/DET/FUS proteins was initially defined by their ability to repress photomorphogenesis in Arabidopsis. It was proposed that this regulation be mediated by targeting degradation of key cellular regulators that promote photomorphogenesis. Among them, COP1 and the COP9 signalosome have been hypothesized to fulfill the ... More
A new class of regulatory genes underlying the cause of pear-shaped tomato fruit.
Authors Liu Jiping; Van Eck Joyce; Cong Bin; Tanksley Steven D;
JournalProc Natl Acad Sci U S A
PubMed ID12242331
A common, recurring theme in domesticated plants is the occurrence of pear-shaped fruit. A major quantitative trait locus (termed ovate) controlling the transition from round to pear-shaped fruit has been cloned from tomato. OVATE is expressed early in flower and fruit development and encodes a previously uncharacterized, hydrophilic protein with ... More
Molecular characterization and role of bovine upstream stimulatory factor 1 and 2 in the regulation of the prostaglandin G/H synthase-2 promoter in granulosa cells.
AuthorsSayasith K, Bouchard N, Sawadogo M, Lussier JG, Sirois J,
JournalJ Biol Chem
PubMed ID14660559
The transcriptional activation of the prostaglandin G/H synthase-2 (PGHS-2) gene in granulosa cells is required for ovulation. To directly study the ability of upstream stimulatory factor 1 (USF1) and USF2 to trans-activate the bovine PGHS-2 promoter in granulosa cells, USF1 or USF2 expression vectors were cotransfected with the PGHS-2/luciferase (LUC) ... More
Characterization of Drosophila hemoglobin. Evidence for hemoglobin-mediated respiration in insects.
In contrast to previous assumptions, the fruit fly Drosophila melanogaster possesses hemoglobin. This respiratory protein forms a monomer of about 17 kDa that is not exported into the hemolymph. Recombinant Drosophila hemoglobin displays a typical hexacoordinated deoxy spectrum and binds oxygen with an affinity of 0.12 torr. Four different hemoglobin ... More
A biochemical and molecular characterization of LEP1, an extensin peroxidase from lupin.
AuthorsPrice NJ, Pinheiro C, Soares CM, Ashford DA, Ricardo CP, Jackson PA,
JournalJ Biol Chem
PubMed ID12882982
An analysis of apoplastic extensin cross-linking activity in vegetative organs of Lupinus albus indicated that leaves contained the highest specific activity. Assays of peroxidases fractionated from this material demonstrated that this activity could be largely attributed to a soluble and apoplastic 51-kDa peroxidase, denoted LEP1. Relative to other purified peroxidases, ... More
Role of DIVARICATA in the control of dorsoventral asymmetry in Antirrhinum flowers.
Authors Galego Lisete; Almeida Jorge;
JournalGenes Dev
PubMed ID11937495
Dorsoventral asymmetry of the Antirrhinum corolla depends on expression of the CYC and DICH genes in dorsal petals. One role of these genes is to inhibit DIVARICATA (DIV), a determinant of ventral identity. Therefore, in cyc;dich double mutants ventral identity spreads all around the flower. We show that DIV encodes ... More
Identification of a cis element for tissue-specific alternative splicing of chloroplast ascorbate peroxidase pre-mRNA in higher plants.
Alternative splicing events in the 3'-terminal region of chloroplast ascorbate peroxidase (chlAPX) pre-mRNA in spinach and tobacco, which produced four types of mRNA variants, one form (tAPX-I) encoding thylakoid-bound APX (tAPX) and three forms (sAPX-I, -II, and -III) encoding stromal APX (sAPX), were regulated in a tissue-specific manner. The ratio ... More
Alternative promoter identified between a hypermethylated upstream region of repetitive elements and a CpG island in human ABO histo-blood group genes.
We have studied the expression of human histo-blood group ABO genes during erythroid differentiation, using an ex vivo culture of AC133(-)CD34(+) cells obtained from peripheral blood. 5'-Rapid amplification of cDNA ends analysis of RNA from those cells revealed a novel transcription start site, which appeared to mark an alternative starting ... More
A virus discovery method incorporating DNase treatment and its application to the identification of two bovine parvovirus species.
Authors Allander T; Emerson S U; Engle R E; Purcell R H; Bukh J;
JournalProc Natl Acad Sci U S A
PubMed ID11562506
Identification of previously unrecognized viral agents in serum or plasma samples is of great medical interest but remains a major challenge, primarily because of abundant host DNA. The current methods, library screening or representational difference analysis (RDA), are very laborious and require selected sample sets. We have developed a simple ... More
Two myostatin genes are differentially expressed in myotomal muscles of the trout (Oncorhynchus mykiss).
Authors Rescan P Y; Jutel I; Rallière C;
JournalJ Exp Biol
PubMed ID11707501
Myostatin (GDF8) has been shown to be a major genetic determinant of skeletal muscle growth in mammals. In this study, we report the cloning of two trout cDNAs that encode two distinct myostatin-related proteins. The presence in this fish species of two myostatin genes (Tmyostatin 1 and Tmyostatin 2) probably ... More
The WNT7b promoter is regulated by TTF-1, GATA6, and Foxa2 in lung epithelium.
Authors Weidenfeld Joel; Shu Weiguo; Zhang Lili; Millar Sarah E; Morrisey Edward E;
JournalJ Biol Chem
PubMed ID11914369
In this study, we find that WNT7b is the only member of the WNT family of autocrine/paracrine signaling molecules whose expression in the lung is restricted to the airway epithelium during embryonic development. To study the transcriptional mechanisms that underlie this restricted pattern of WNT7b expression, we isolated the proximal ... More
Cloning of a novel retinoid-inducible serine carboxypeptidase from vascular smooth muscle cells.
Authors Chen J; Streb J W; Maltby K M; Kitchen C M; Miano J M;
JournalJ Biol Chem
PubMed ID11447226
Retinoids block smooth muscle cell (SMC) proliferation and attenuate neointimal formation after vascular injury, presumably through retinoid receptor-mediated changes in gene expression. To identify target genes in SMC whose encoded proteins could contribute to such favorable biological effects, we performed a subtractive screen for retinoid-inducible genes in cultured SMC. Here, ... More
Molecular cloning of a novel chaperone-like protein induced by rhabdovirus infection with sequence similarity to the bacterial extracellular solute-binding protein family 5.
Authors Cho Wha Ja; Yoon Won Joon; Moon Chang Hoon; Cha Seung Ju; Song Hebok; Cho Hong Rae; Jang Soo Jin; Chung Dae Kyun; Jeong Choon Soo; Park Jeong Woo;
JournalJ Biol Chem
PubMed ID12183466
Previously we demonstrated that a novel stress protein is induced in fish cells by the infection of a fish rhabdovirus (Cho W. J., Cha, S. J., Do, J. W., Choi, J. Y., Lee, J. Y., Jeong, C. S., Cho, K. J., Choi, W. S., Kang, H. S., Kim, H. D., ... More
The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia.
Authors Touz Maria C.; Nores Maria J.; Slavin Ileana; Carmona Carlos; Conrad John T.; Mowatt Michael R.; Nash Theodore E.; Coronel Carlos E.; Lujan Hugo D.;
JournalJ Biol Chem
PubMed ID11773053
Giardia is an intestinal parasite that belongs to the earliest diverging branch of the eukaryotic lineage of descent. Giardia undergoes adaptation for survival outside the host's intestine by differentiating into infective cysts. Encystation involves the synthesis and transport of cyst wall constituents to the plasma membrane for release and extracellular ... More
We used an autoimmune serum from a patient with discoid lupus erythematosus to clone a cDNA of 2808 base pairs. Its open reading frame of 2079 base pairs encodes a predicted polypeptide of 693 amino acids named CDA1 (cell division autoantigen-1). CDA1 has a predicted molecular mass of 79,430 Daltons ... More
Differential characteristics and subcellular localization of two starch-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L.
Starch-branching enzymes (SBE) have a dominant role for amylopectin structure as they define chain length and frequency of branch points. We have previously shown that one of the SBE isoforms of kidney bean (Phaseolus vulgaris L.), designated PvSBE2, has a molecular mass (82 kDa) significantly smaller than those reported for ... More