BLOCK-iT™ 荧光寡核苷酸，用于脂质转染
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引用和文献 (4)
Invitrogen™

BLOCK-iT™ 荧光寡核苷酸，用于脂质转染

BLOCK-iT™ 荧光寡核苷酸提供了：• 一种强大、易于检测到的 FITC 信号，该信号可指示转染效率• 化学修饰，可形成一种相对于标记 siRNA 更明确、更具持久性的细胞信号•了解更多信息
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货号数量
20132 x 125 μL
货号 2013
价格（CNY)
2,372.00
Each
添加至购物车
数量:
2 x 125 μL
价格（CNY)
2,372.00
Each
添加至购物车
BLOCK-iT™ 荧光寡核苷酸提供了：

• 一种强大、易于检测到的 FITC 信号，该信号可指示转染效率
• 化学修饰，可形成一种相对于标记 siRNA 更明确、更具持久性的细胞信号
• Stealth RNAi™ siRNA 和传统 siRNA 分子转染效率的已证明相关性

这使得 BLOCK-iT™ 荧光寡核苷酸成为 RNAi 实验转染效率的理想指示剂。可以提供两种配置的 BLOCK-iT™ 荧光寡核苷酸：一种设计用于 20 µM 下的脂质介导转染 (2 x 125µL)，一种设计用于 1 mM 下的电穿孔 (75 µL)。
仅供科研使用。不可用于诊断程序。
规格
适用于（应用）脂质转染
标签或染料FITC（荧光素）
产品类型寡核苷酸
数量2 x 125 μL
产品线BLOCK-iT
Unit SizeEach
内容与储存
用于脂质转染的 BLOCK-iT™ 荧光寡核苷酸以 2 x 125 µL 20 µM 管形式提供。在 -20°C 下储存。

常见问题解答 (FAQ)

在Stealth RNAi siRNA实验中我应该使用什么阳性对照和阴性对照？

我们为Stealth siRNA提供多个阳性和阴性对照：

Stealth阳性对照有：
- Stealth RNAi siRNA Actin阳性对照（人）（货号12935141, 250 µL）
- Stealth RNAi siRNA Cyclophilin B阳性对照（人）（货号12935142, 250 µL）
- Stealth RNAi siRNA GAPDH阳性对照（人）（货号12935140, 250 µL）
- Stealth RNAi siRNA β-Lactamase报告基因对照（货号12935148, 250 µL）

- Stealth RNAi siRNA GFP报告基因对照（货号12935145, 250 µL）
- Stealth RNAi siRNA Luciferase报告基因对照（货号12935146, 250 µL）

Stealth阴性对照有：
提供高(55-65% GC), 中(45-55% GC), 或低(35-45% GC)三种GC含量的双链siRNA对照：
- 高GC含量的Stealth RNAi siRNA阴性对照（货号12935400, 250 µL）
- 高GC含量的Stealth RNAi siRNA阴性对照#2（货号12935114, 250 µL）
- 高GC含量的Stealth RNAi siRNA阴性对照#3（货号12935115, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照（货号12935300, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照#2（货号12935112, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照#3（货号12935113, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照（货号12935200, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照#2（货号12935110, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照#3（货号12935111, 250 µL）
- Stealth RNAi siRNA阴性对照试剂盒（货号12935100，包含每种GC含量阴性对照各20 µM各250 µL）
- 对于随机序列阴性对照，您可以使用BLOCK-iT RNAi Designer对您的siRNA或Stealth RNAi序列进行打乱。

您也可以使用我们的BLOCK-iT AlexaFluorRed Fluorescent Control（货号14750100）或BLOCK-iTFluorescent Oligo（货号2013, 13750062）作为转染的对照。

What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?

We offer several negative and positive controls for Stealth siRNA:

For Stealth Positive Control:
- Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
- Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
- Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
- Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)

For Stealth Negative Control:
Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:

- Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
- Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
- For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.

You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Can I use the BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100)?

  • We do not recommend using BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100). BLOCK-iT Alexa Fluor Red Fluorescent Control (Cat. No. 14750100) should be used to assess transfection efficiency with Lipofectamine RNAiMAX Reagent.
  • BLOCK-iT Fluorescent Oligo, for lipid transfection is optimized for use with Lipofectamine 2000 Transfection Reagent and is not recommended for Lipofectamine RNAiMAX Reagent.


    • Find additional tips, troubleshooting help, and resources within our RNAi Support Center

    引用和文献 (4)

    引用和文献
    Abstract
    RNAi specificity: how big of an issue is it?
    Authors:Samarsky DA, Welch PJ,
    Journal:Pharmacogenomics
    PubMed ID:15723598
    RNAi techniques hold great promise for science, from basic research all the way to human therapeutics, and our understanding of the underlying mechanisms have allowed us to design better and more effective compounds. Fortunately, much has also been learned about nonspecific effects, such as off-target and stress responses. These observations and discoveries have led us to improved specificity ... More
    CD147 is a regulatory subunit of the gamma-secretase complex in Alzheimer's disease amyloid beta-peptide production.
    Authors:Zhou S, Zhou H, Walian PJ, Jap BK,
    Journal:Proc Natl Acad Sci U S A
    PubMed ID:15890777
    'gamma-Secretase is a membrane protein complex that cleaves the beta-amyloid precursor protein (APP) within the transmembrane region, after prior processing by beta-secretase, producing amyloid beta-peptides Abeta(40) and Abeta(42). Errant production of Abeta-peptides that substantially increases Abeta(42) production has been associated with the formation of amyloid plaques in Alzheimer''s disease patients. ... More
    Differentiation of mouse embryonic stem cells following RNAi-mediated silencing of OCT4 and Nanog.
    Authors:Hough SR, Clements I, Welch PJ, Wiederholt KA,
    Journal:Stem Cells
    PubMed ID:16456133
    RNAi holds great promise as a tool to study the basic biology of stem cells or to direct differentiation in a specific manner. Barriers to achieving efficient and specific gene silencing in RNAi experiments include limitations in transfection efficiency and in the efficacy and specificity of RNAi silencing effectors. Here, ... More
    IpgB1 is a novel Shigella effector protein involved in bacterial invasion of host cells. Its activity to promote membrane ruffling via Rac1 and Cdc42 activation.
    Authors:Ohya K, Handa Y, Ogawa M, Suzuki M, Sasakawa C,
    Journal:J Biol Chem
    PubMed ID:15849186
    Shigella, the causative agent of bacillary dysentery, is capable of inducing the large scale membrane ruffling required for the bacterial invasion of host cells. Shigella secrete a subset of effectors via the type III secretion system (TTSS) into the host cells to induce membrane ruffling. Here, we show that IpgB1 ... More